Detailed information of TA system TA143478

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 1729965..1730536
• Replicon: chromosome
• Accession: NZ_CP046058
• Organism: Microcystis aeruginosa NIES-298

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: D3800_RS08280
• Protein ID: WP_103112813.1
• Coordinates: 1730204..1730536 (+)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: D3800_RS08275
• Protein ID: WP_103112812.1
• Coordinates: 1729965..1730207 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D3800_RS08265	1728401..1729063	+	663	WP_002738780.1	potassium channel family protein	-
D3800_RS08270	1729097..1729873	+	777	WP_103112811.1	DUF2092 domain-containing protein	-
D3800_RS08275	1729965..1730207	+	243	WP_103112812.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
D3800_RS08280	1730204..1730536	+	333	WP_103112813.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
D3800_RS08285	1730718..1731593	+	876	WP_069474485.1	hypothetical protein	-
D3800_RS08290	1731590..1734652	-	3063	WP_103112814.1	DEAD/DEAH box helicase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-109)

Antitoxin

• Antitoxin PemI-like (1-80)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 12616.71 Da
• Isoelectric Point: 10.1582

>T143478 WP_103112813.1 NZ_CP046058:1730204-1730536 [Microcystis aeruginosa NIES-298]
VTYIPERGDFLRLSFDPQIGHEQMGNRPALVVSHIDFNRKIGFAFVCPVSNTQRQNPFYVKIPDGEAVTGVIMVDQLRSL
DFRARKASFIGKCPERLLQDVLRRIKPILF

Nucleotide

• Length: 333 bp

>T143478 NZ_CP061108:2106769-2106872 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9041.36 Da
• Isoelectric Point: 4.0460

>AT143478 WP_103112812.1 NZ_CP046058:1729965-1730207 [Microcystis aeruginosa NIES-298]
MKSQIRRWGNSLAIRIPKYAVEALNLNPNDVVECSVENGKLVIELIQALPELSLEELLAEVVESPEPEVDWGRPMGEEVW

Nucleotide

• Length: 243 bp

>AT143478 NZ_CP061108:c2106910-2106639 [Escherichia coli]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTGAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT