Detailed information of TA system TA143477

Bioinformatically predicted

Overview

TA module

• Type: VII
• Classification (family/domain): HepT-MntA/HepT(toxin)
• Location: 1660162..1661021
• Replicon: chromosome
• Accession: NZ_CP046058
• Organism: Microcystis aeruginosa NIES-298

Toxin (Protein)

• Gene name: hepT
• Uniprot ID: I4HG17
• Locus tag: D3800_RS08000
• Protein ID: WP_002791882.1
• Coordinates: 1660623..1661021 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: mntA
• Uniprot ID: I4HG15
• Locus tag: D3800_RS07995
• Protein ID: WP_002791881.1
• Coordinates: 1660162..1660581 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D3800_RS07980	1657298..1659043	+	1746	WP_202935276.1	IS1634 family transposase	-
D3800_RS07985	1659115..1659306	+	192	WP_103112784.1	hypothetical protein	-
D3800_RS07990	1659652..1659999	+	348	WP_002742891.1	hypothetical protein	-
D3800_RS07995	1660162..1660581	+	420	WP_002791881.1	nucleotidyltransferase domain-containing protein	Antitoxin
D3800_RS08000	1660623..1661021	+	399	WP_002791882.1	DUF86 domain-containing protein	Toxin
D3800_RS08005	1661014..1661472	+	459	WP_103112785.1	nucleotidyltransferase domain-containing protein	-
D3800_RS08010	1661476..1661700	+	225	WP_103112786.1	DUF86 domain-containing protein	-
D3800_RS08015	1661693..1665154	+	3462	WP_103112787.1	type I-D CRISPR-associated protein Cas10d/Csc3	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease HepT-like (1-117)

Antitoxin

• Polymerase beta, nucleotidyltransferase (6-101)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15326.56 Da
• Isoelectric Point: 4.6345

>T143477 WP_002791882.1 NZ_CP046058:1660623-1661021 [Microcystis aeruginosa NIES-298]
MNQYLAELSEYGSITLEDYRTLRERQLAIERLIQLIVQTGIDINYQILKCLDIESPNNARDALFQIVELGILEEHLAVQL
AESIKLRNLLVHLYKKIDPDIVHSSIANILRDYPRYQRSIVQYLDSLEAENG

Nucleotide

• Length: 399 bp

>T143477 NZ_CP061108:1850228-1850482 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 16150.65 Da
• Isoelectric Point: 5.0933

>AT143477 WP_002791881.1 NZ_CP046058:1660162-1660581 [Microcystis aeruginosa NIES-298]
MNRLVEKITAIVEKLPNLKLLILFGSRARGEHKPDSDWDFAVLYEERSAQKDISSLLKIYTLLEQALEIPEDKIDVIDLK
ECSPILAHYVARDGQLLYERETGIFEGFKEKFLMNPEESKALYRQLRNNLEISLREKGV

Nucleotide

• Length: 420 bp

>AT143477 NZ_CP061108:1849980-1850231 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATATAACTCCCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Structures

Toxin

• AlphaFold DB: I4HG17

Antitoxin

• AlphaFold DB: I4HG15