Detailed information of TA system TA143473

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 241895..242508
• Replicon: chromosome
• Accession: NZ_CP046058
• Organism: Microcystis aeruginosa NIES-298

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: I4HQS4
• Locus tag: D3800_RS01200
• Protein ID: WP_002793526.1
• Coordinates: 241895..242281 (-)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: I4GD21
• Locus tag: D3800_RS01205
• Protein ID: WP_002775288.1
• Coordinates: 242281..242508 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D3800_RS01175	237577..237984	+	408	WP_103112534.1	hypothetical protein	-
D3800_RS01180	238027..238278	-	252	WP_002750923.1	hypothetical protein	-
D3800_RS01185	238284..239042	-	759	WP_103112535.1	hypothetical protein	-
D3800_RS01190	239073..239465	-	393	WP_103112536.1	Mo-dependent nitrogenase C-terminal domain-containing protein	-
D3800_RS01195	240045..241802	-	1758	WP_103112537.1	arginine--tRNA ligase	-
D3800_RS01200	241895..242281	-	387	WP_002793526.1	type II toxin-antitoxin system VapC family toxin	Toxin
D3800_RS01205	242281..242508	-	228	WP_002775288.1	DUF2281 domain-containing protein	Antitoxin
D3800_RS01210	242550..243029	-	480	WP_002750935.1	2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase	-
D3800_RS01215	243026..243193	-	168	WP_162205044.1	hypothetical protein	-
D3800_RS01220	243432..246245	-	2814	WP_103112538.1	preprotein translocase subunit SecA	-
D3800_RS01225	246526..246888	+	363	WP_103112539.1	four helix bundle protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• VapC-like Sll0205 protein, PIN domain (5-127)

Antitoxin

• Domain of unknown function DUF2281 (6-74)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14836.14 Da
• Isoelectric Point: 4.8781

>T143473 WP_002793526.1 NZ_CP046058:c242281-241895 [Microcystis aeruginosa NIES-298]
MTSFLLDTHTFIWLTENDPNLPNNLREEIDFAPEVYVSIVSLWEIAIKLNLGKLALQKSYETIETKLEASDITLLSISFS
DTLKICTLPLYHRDPFDRMLIAQSLNRSLILISKDTKFDAYNVPRKWT

Nucleotide

• Length: 387 bp

>T143473 NZ_CP061108:1049829-1050164 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8847.08 Da
• Isoelectric Point: 4.1064

>AT143473 WP_002775288.1 NZ_CP046058:c242508-242281 [Microcystis aeruginosa NIES-298]
MNIDTKILETVETMPEFLKIELLHYAEYLIANYQSDVIIEKPSLRKRRSGILQGTFTLPLSDDFDESLEDFQEYM

Nucleotide

• Length: 228 bp

>AT143473 NZ_CP061108:1049581-1049829 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Structures

Toxin

• AlphaFold DB: I4HQS4

Antitoxin

• AlphaFold DB: I4GD21