Detailed information of TA system TA143469

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pmenTA/darT(toxin)
• Location: 1607657..1609371
• Replicon: chromosome
• Accession: NZ_CP046056
• Organism: Venatoribacter cucullus strain ASxL5

Toxin (Protein)

• Gene name: pmenT
• Uniprot ID: -
• Locus tag: GJQ55_RS07595
• Protein ID: WP_228344379.1
• Coordinates: 1608727..1609371 (-)
• Length: 215 a.a.

Antitoxin (Protein)

• Gene name: pmenA
• Uniprot ID: -
• Locus tag: GJQ55_RS07590
• Protein ID: WP_228344378.1
• Coordinates: 1607657..1608727 (-)
• Length: 357 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GJQ55_RS07570 (GJQ55_07510)	1602820..1603233	-	414	WP_228344375.1	type II toxin-antitoxin system HicB family antitoxin	-
GJQ55_RS07575 (GJQ55_07515)	1603248..1603427	-	180	Protein_1480	type II toxin-antitoxin system HicA family toxin	-
GJQ55_RS07580 (GJQ55_07520)	1603670..1604383	-	714	WP_228344376.1	SprT family zinc-dependent metalloprotease	-
GJQ55_RS07585 (GJQ55_07525)	1604383..1607496	-	3114	WP_228344377.1	HsdR family type I site-specific deoxyribonuclease	-
GJQ55_RS07590 (GJQ55_07530)	1607657..1608727	-	1071	WP_228344378.1	macro domain-containing protein	Antitoxin
GJQ55_RS07595 (GJQ55_07535)	1608727..1609371	-	645	WP_228344379.1	DUF4433 domain-containing protein	Toxin
GJQ55_RS07600 (GJQ55_07540)	1609376..1609966	-	591	WP_228344380.1	DUF4433 domain-containing protein	-
GJQ55_RS07605 (GJQ55_07545)	1609976..1611472	-	1497	WP_228344381.1	restriction endonuclease subunit S	-
GJQ55_RS07610 (GJQ55_07550)	1611469..1613985	-	2517	WP_228344382.1	type I restriction-modification system subunit M	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• ssDNA thymidine ADP-ribosyltransferase, DarT (14-214)

Antitoxin

• Macro domain (18-129)

Sequences

Toxin

Protein

• Length: 215 a.a.
• Molecular weight: 24516.81 Da
• Isoelectric Point: 7.5416

>T143469 WP_228344379.1 NZ_CP046056:c1609371-1608727 [Venatoribacter cucullus]
MLYTSLNPQKALIWRIVHRDNLPWILANGLYAGNHAQHSDNWVSIGNPELITKRGSHRVPVRPFGVLNDYVPFYFTPFSP
MLRNIQTGWGGITRRSNDEIVILVSSIHRLEQAGLPYVFTDSHAYYQWANFYTCAAHLDKIDWPLLQRRDFARSVDDPAK
IERYQAEALVYQHCPVTALIGIITHNDTDRADVQAQVTAQGLGLQVEARPGWYF

Nucleotide

• Length: 645 bp

>T143469 NZ_CP061108:329394-329921 [Escherichia coli]
ATGGATGATCTGACGATAGAGATTCTGACCGATGATGCAGATTATGATCTACAGCGATTCGACTGCGGCGAGGAAGCGTT
AAATCTCTTTCTGACGACACATCTCGTTCGTCAACATCGCAACAAAATTCTGCGAGCGTATATCCTTTGTCGCAACACTC
CAGAACGTCAGGTGCTGGGATATTACACATTATGCGGCAGTTGTTTTGAACGAGCCGCATTGCCCTCGAAATCGAAACAG
AAAAAAATTCCCTACAAAAATATTCCCAGCGTTACTCTTGGGCGTCTGGCAATTGATCGTTCATTACAGGGGCAGGGATG
GGGAGCAACACTGGTTGCTCATGCCATGAAAGTCGTCTGGTCAGCCTCTTTAGCGGTAGGTATTCACGGTCTTTTTGTCG
AGGCGCTGAATAAAAAAGCCCATACGTTTTATCAATCGCTGGGCTTTATCCCGTTGGTCGGAGAAAACGAAAATGCGTTA
TTTTTCCCAACCAAATCCATCGAACTGCTTTTCACACAGAGCGATTAA

Antitoxin

Protein

• Length: 357 a.a.
• Molecular weight: 39975.76 Da
• Isoelectric Point: 6.0547

>AT143469 WP_228344378.1 NZ_CP046056:c1608727-1607657 [Venatoribacter cucullus]
MIEYTQGNLLEAPVEALVNTVNTVGVMGKGIALMFKERFPANMQAYAQACKQQQVQTGHMFITETGELMGPRWIVNFPTK
QHWRGNSRMEWVESGLQDLRRFIVEQQVQSIAIPPLGAGNGGLSWADVKPRIEAALGDIPGVQVLVYEPTAAYQNVAKRN
GVQNLTEARALVAELVRRYWLLGIDCSLLEIQKLAWFLQRAIQLQGLPDKLQLNFVANNYGPYANNLDHLLNALDGSYLK
AEKRIPDSDPLDVIWFRDETRDKVQAYLNSAAKDYVPALEMASAMIEGFESPYGMELLATVDWLLTREGVNANVADVRAA
LQHWPAGARWAQRKNRLFDDNSLHIAIERLARFQAA

Nucleotide

• Length: 1071 bp

>AT143469 NZ_CP061108:329121-329387 [Escherichia coli]
ATGTCTGCTGTTAAAAAGCAGCGTATCGATCTGCGTTTAACTGACGATGACAAAAGTATGATTGAGGAAGCGGCAGCGAT
ATCTAATCAGTCCGTCAGCCAATTTATGTTGAACAGCGCTTCGCAGCGGGCTGCGGAAGTGATTGAACAGCATCGGCGGG
TGATCCTCAATGAGGAATCCTGGACGCGGGTGATGGATGCGCTGAGTAATCCACCGTCACCTGGTGAAAAGCTAAAACGT
GCGGCAAAACGTCTTCAGGGAATGTAA