Detailed information of TA system TA143467

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 2114031..2114827
• Replicon: chromosome
• Accession: NZ_CP046052
• Organism: Methylocystis heyeri strain H2

Toxin (Protein)

• Gene name: TacT3
• Uniprot ID: -
• Locus tag: H2LOC_RS09665
• Protein ID: WP_136496213.1
• Coordinates: 2114031..2114555 (-)
• Length: 175 a.a.

Antitoxin (Protein)

• Gene name: TacA3
• Uniprot ID: -
• Locus tag: H2LOC_RS09670
• Protein ID: WP_154331614.1
• Coordinates: 2114552..2114827 (-)
• Length: 92 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H2LOC_RS09650	2109221..2110990	+	1770	WP_136496210.1	hypothetical protein	-
H2LOC_RS09655	2111157..2111426	+	270	WP_136496211.1	30S ribosomal protein S15	-
H2LOC_RS09660	2111690..2113858	+	2169	WP_136496212.1	polyribonucleotide nucleotidyltransferase	-
H2LOC_RS09665	2114031..2114555	-	525	WP_136496213.1	GNAT family N-acetyltransferase	Toxin
H2LOC_RS09670	2114552..2114827	-	276	WP_154331614.1	DUF1778 domain-containing protein	Antitoxin
H2LOC_RS09675	2115111..2115680	+	570	WP_136496215.1	hypothetical protein	-
H2LOC_RS09680	2115854..2119309	-	3456	WP_136496216.1	double-strand break repair helicase AddA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Vibrio phage ICP1, Orf50 (10-85)

Sequences

Toxin

Protein

• Length: 175 a.a.
• Molecular weight: 19103.79 Da
• Isoelectric Point: 7.7234

>T143467 WP_136496213.1 NZ_CP046052:c2114555-2114031 [Methylocystis heyeri]
MTEISWEESPLGKQHDRNAFNCGDDDLNTYLKRYARQNHESGGAKCFVATPRGEPAHILGFYTLCPASIKFDRAPAVMTR
GGLGRYEVPVFRLGRLAVDQSLHGRGLGGSLLLRAGERCMMVAEQVGGVAVLIDAKDDRAAQWYESYGAVRFNDAPRSLM
LLFAAVLDALKQSP

Nucleotide

• Length: 525 bp

>T143467 NZ_CP061103:c59133-58831 [Escherichia coli]
ATGACCAAAAAAATTAACATAAAGGATTTCAGGGATGCGTGGCTTGATGATTTTTTTGAATTTTCAACACCACATAGAAA
GATACCTCCTGATATTCATATGACATTGTCACGGAAGTTGGACATTATCAATGCCGCAACTACCTGTAAGGATTTAAGAT
CACCACCAGGTAATCGGTATGAGGAACTGTCAGGGAAGCTAAATGGCTATTCATCAGTAAGGGTGAATAAGCAATATAGG
TTAATTTTTAAGTGGGTTAACGGAAAGGCCGAGGACTTGTATCTCGACCCTCACAAATACTAA

Antitoxin

Protein

• Length: 92 a.a.
• Molecular weight: 10331.06 Da
• Isoelectric Point: 8.6370

>AT143467 WP_154331614.1 NZ_CP046052:c2114827-2114552 [Methylocystis heyeri]
MPREHVENGRVELRLKPEDKAVLARAAAIERLDLTSFILRSVLPRAKEVISDYETLQLSERDSLLILDLLENPPKANERL
IRAAKAGFVLK

Nucleotide

• Length: 276 bp

>AT143467 NZ_CP061103:c58760-58422 [Escherichia coli]
ATGAAACAGGCAACCAGAAAACCGACGACCGTAGGTGATATCCTGCTGTACGAATACCTGGAGCCGTTAGAGCTGAAGAT
CAACGAGTTAGCAGAAATACTTCATGTTCATCGTAACACCGTAAGTGCTCTTGTTAATAACAATCGTAAGCTAACGATGG
ATATGGCATATCGCCTGGCAAAAGCATTCGATACTTCTGTAGATTTTTGGATTAATCTTCAGACCGCAGTAGATCTGTGG
GAAGTCGAAAATGATATGCGCGTTCAGGAAGAGTTAAGTCGTATCAATACTGCTGAAAAATTTATTTCTCAGCGGAACCT
GAATAAAAAAGCAGCCTGA