Detailed information of TA system TA143465

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 44851..45428
• Replicon: plasmid pDY.F1.2
• Accession: NZ_CP046050
• Organism: Proteus mirabilis strain DY.F1.2

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: U5N651
• Locus tag: GJR68_RS18605
• Protein ID: WP_023159957.1
• Coordinates: 45096..45428 (+)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: GJR68_RS18600
• Protein ID: WP_096864992.1
• Coordinates: 44851..45096 (+)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GJR68_RS18560	39873..40217	+	345	WP_023159966.1	single-stranded DNA-binding protein	-
GJR68_RS18565	40210..40632	+	423	WP_172688766.1	hypothetical protein	-
GJR68_RS18570	40641..41033	+	393	WP_096864995.1	hypothetical protein	-
GJR68_RS18575	41313..41792	+	480	WP_048821772.1	Hcp family type VI secretion system effector	-
GJR68_RS18580	41801..42380	+	580	Protein_44	hypothetical protein	-
GJR68_RS18585	43140..43508	+	369	WP_096864994.1	hypothetical protein	-
GJR68_RS18590	43606..43833	-	228	WP_096864993.1	DNA partition complex ParG	-
GJR68_RS18595	43942..44565	-	624	WP_023159958.1	AAA family ATPase	-
GJR68_RS18600	44851..45096	+	246	WP_096864992.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
GJR68_RS18605	45096..45428	+	333	WP_023159957.1	endoribonuclease MazF	Toxin
GJR68_RS18610	45459..45839	+	381	WP_096864991.1	hypothetical protein	-
GJR68_RS18615	45925..46068	-	144	WP_071547955.1	type I toxin-antitoxin system Hok family toxin	-
GJR68_RS18620	46433..47230	-	798	WP_172688767.1	hypothetical protein	-
GJR68_RS18625	47832..48587	+	756	WP_159287658.1	site-specific integrase	-
GJR68_RS18630	48601..49419	+	819	WP_023159955.1	DUF4365 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	sul2 / floR / dfrA14 / ARR-3 / blaOXA-10 / ant(3'')-Ia / qacE / sul1 / blaNDM-1 / mph(E) / msr(E) / aac(3)-IVa / aph(4)-Ia	-	1..165918	165918

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (9-107)

Antitoxin

• SpoVT-AbrB domain (7-49)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 12097.88 Da
• Isoelectric Point: 7.0813

>T143465 WP_023159957.1 NZ_CP046050:45096-45428 [Proteus mirabilis]
MVSRYVPDSGDLIWIDFDPVEGHEQGGHRPAVVLSPFAYNNKVGLLLCVPCTTKGKGYPFECELSNERDSYALADQVTCV
DWRARKVTKKGKVEASELAEIKAKAKALIG

Nucleotide

• Length: 333 bp

>T143465 NZ_CP061102:c68396-68238 [Escherichia coli]
ATGAAACTACCACGCAGCTCTCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGATACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 8800.23 Da
• Isoelectric Point: 5.0508

>AT143465 WP_096864992.1 NZ_CP046050:44851-45096 [Proteus mirabilis]
MAQVTVKKWGNSPSVRLPVAIMQKAALSVDDTVEIAVEEGRIIITPVKAVEYSLENLLAGITPENIHEKVDFGARTGKEL
I

Nucleotide

• Length: 246 bp

>AT143465 NZ_CP061102:c68659-68461 [Escherichia coli]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGGATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAGGA
CAAAAGCCCCGTAGTTAATTTTTCATTAACCCACGAGGC

Structures

Toxin

• AlphaFold DB: A0A2P1BQ75