Detailed information of TA system TA143464

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mosAT/AbiEii-AbiEi
• Location: 1149516..1151238
• Replicon: chromosome
• Accession: NZ_CP046049
• Organism: Proteus mirabilis strain DY.F1.2

Toxin (Protein)

• Gene name: mosT
• Uniprot ID: A0A0U2S2I2
• Locus tag: GJR68_RS05450
• Protein ID: WP_064497040.1
• Coordinates: 1150300..1151238 (+)
• Length: 313 a.a.

Antitoxin (Protein)

• Gene name: mosA
• Uniprot ID: A0A934MVJ4
• Locus tag: GJR68_RS05445
• Protein ID: WP_000095577.1
• Coordinates: 1149516..1150307 (+)
• Length: 264 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GJR68_RS05415	1145189..1145470	+	282	WP_000433891.1	type IV conjugative transfer system protein TraL	-
GJR68_RS05420	1145467..1146093	+	627	WP_064497042.1	pilus assembly protein	-
GJR68_RS05425	1146077..1146973	+	897	WP_064497055.1	type-F conjugative transfer system secretin TraK	-
GJR68_RS05430	1146976..1148265	+	1290	WP_064497041.1	TraB/VirB10 family protein	-
GJR68_RS05435	1148340..1148912	+	573	WP_024008834.1	type IV conjugative transfer system lipoprotein TraV	-
GJR68_RS05440	1148909..1149295	+	387	WP_032469918.1	hypothetical protein	-
GJR68_RS05445	1149516..1150307	+	792	WP_000095577.1	type IV toxin-antitoxin system AbiEi family antitoxin	Antitoxin
GJR68_RS05450	1150300..1151238	+	939	WP_064497040.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
GJR68_RS05455	1151370..1152062	+	693	WP_001228924.1	DsbC family protein	-
GJR68_RS05460	1152062..1154461	+	2400	WP_064497039.1	type IV secretion system protein TraC	-
GJR68_RS05465	1154454..1154801	+	348	WP_064497038.1	hypothetical protein	-
GJR68_RS05470	1154785..1155297	+	513	WP_175215521.1	S26 family signal peptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	sul2 / aph(4)-Ia / aac(3)-IVa / blaCTX-M-65 / floR / aph(6)-Id / aph(3'')-Ib / tet(C) / aph(3')-Ia / aac(6')-Ib-cr / blaOXA-1 / catB3 / ARR-3 / qacE / sul1 / ere(A) / aadA2 / tet(J)	htpB	1028454..1300985	272531

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (14-272)

Antitoxin

• Transcriptional regulator, AbiEi 3 antitoxin, Type IV TA system (99-250)

Sequences

Toxin

Protein

• Length: 313 a.a.
• Molecular weight: 35542.81 Da
• Isoelectric Point: 4.9912

>T143464 WP_064497040.1 NZ_CP046049:1150300-1151238 [Proteus mirabilis]
MDKDSPYYKQVSLLIRMLPVVATETVFALKGGTAINLFVRDFPRLSVDIDLAYLPLEPRDEALINVRAALQRITDRINTQ
PDIRAAFQDNKADELRIIVSSPVATIKIEVSPVARGTLHSAEIMAVQESVEYEFGYAEIQVVSLPDLYGGKLCAAMDRQH
PRDLFDVRMLLGSEGVSREILVGFLTYTLSHPRPINEVMSPNWQPLNEKFQAEFDGMTFEKVECEDLASVRPMMLTSLQK
HFTERDYAFLMSFKRGQPDWALFDYPNAADLPAIRWKLQNINKLAKNQAKHQEQLDKLKQVLDDWLVNANAE

Nucleotide

• Length: 939 bp

>T143464 NZ_CP061102:59824-60222 [Escherichia coli]
ATGCTGAAGTTTATGCTCGATACCAACATCTGCATTTTTACGATAAAGAACAAACCCGCCAGTGTCAGGGAACGTTTTAA
CCTGAACCAGGGGAGAATGTGCATCAGTTCGGTTACTCTGATGGAGGTGATATATGGTGCAGAAAAAAGCCAGATGCCTG
AACGTAATCTCGCTGTGATCGAGGGATTTGTTTCCCGCATTGACGTTCTGGATTACGACGCTGCTGCTGCCACACACACC
GGCCAGATAAGAGCAGAACTTGCCCTTCAGGGACGCCCTGTCGGGCCATTTGATCAAATGATCGCAGGTCATGCCCGCAG
TCGGGGACTGATTATTGTGACTAATAACACCCGGGAATTTGAACGTGTGGGCGGCCTGAGAATTGAAGACTGGAGTTGA

Antitoxin

Protein

• Length: 264 a.a.
• Molecular weight: 30109.49 Da
• Isoelectric Point: 9.5097

>AT143464 WP_000095577.1 NZ_CP046049:1149516-1150307 [Proteus mirabilis]
MSSKINWLVAHTSPGALVLQQWLTENGVSYSLAQKYAQNGWLKKLSSGVYYRPNAQGDIKPTWVDAIQALDVQLGVPVHL
AGLSSLTHQGLSHYLQLNKEQVWICVKNKSSLPKWFREFPYQNWFYCGNHKLEVNPEKDLKRITVKEKELTVSCAELAAY
EVVDAIGKLISFEHVAELFQGLVNLSPRKVQDILERSSSVQANRIFLFLGRYYDHQWVNRIDETRIKLGAGKRQVVEKGR
FDERYQITVPEILSVKKGEQHNG

Nucleotide

• Length: 792 bp

>AT143464 NZ_CP061102:59597-59824 [Escherichia coli]
ATGGAAACCACCGTATTTCTCAGCAACCGCAGCCAGGCGGTCAGACTGCCAAAAGCGGTTGCATTGCCGGAAAACGTAAA
ACGCGTTGAAGTGATTGCTGTCGGACGAACCCGAATCATTACGCCTGCCGGAGAGGCATGGGACGAATGGTTCGACGGAA
ACAGTGTCAGCGCTGATTTTATGGATAACAGGGAACAGCCTGGTATGCAGGAAAGGGAGTCATTCTGA

Structures

Toxin

• AlphaFold DB: A0A0U2S2I2