Detailed information of TA system TA143462

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 430533..432104
• Replicon: chromosome
• Accession: NZ_CP046049
• Organism: Proteus mirabilis strain DY.F1.2

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: GJR68_RS02205
• Protein ID: WP_036908598.1
• Coordinates: 430791..432104 (+)
• Length: 438 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: B4F0T9
• Locus tag: GJR68_RS02200
• Protein ID: WP_004249771.1
• Coordinates: 430533..430790 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GJR68_RS02175	426495..426638	+	144	WP_004246509.1	50S ribosomal protein L34	-
GJR68_RS02180	426656..427015	+	360	WP_004246510.1	ribonuclease P protein component	-
GJR68_RS02185	426979..427239	+	261	WP_012368639.1	membrane protein insertion efficiency factor YidD	-
GJR68_RS02190	427242..428882	+	1641	WP_004246512.1	membrane protein insertase YidC	-
GJR68_RS02195	429020..430384	+	1365	WP_004246513.1	tRNA uridine-5-carboxymethylaminomethyl(34) synthesis GTPase MnmE	-
GJR68_RS02200	430533..430790	+	258	WP_004249771.1	helix-turn-helix domain-containing protein	Antitoxin
GJR68_RS02205	430791..432104	+	1314	WP_036908598.1	type II toxin-antitoxin system HipA family toxin	Toxin
GJR68_RS02210	432507..432971	+	465	WP_004249773.1	DUF1097 domain-containing protein	-
GJR68_RS02215	433156..434085	+	930	WP_004246518.1	metallophosphoesterase	-
GJR68_RS02220	434117..436078	+	1962	WP_012368635.1	hypothetical protein	-
GJR68_RS02225	436132..436935	-	804	WP_004249775.1	energy transducer TonB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (4-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (13-65)

Sequences

Toxin

Protein

• Length: 438 a.a.
• Molecular weight: 49669.80 Da
• Isoelectric Point: 6.6588

>T143462 WP_036908598.1 NZ_CP046049:430791-432104 [Proteus mirabilis]
MASLDVYMNEYYVGIFTKKSSGAHSFQYTEDWVNQPGSRPISLSMPLQLQEYQGDCVYNFFDNLLPDNTEIRNRIVSRYN
ANSNQPFDLLAAIGADTVGALRLLPSGSTPANIKKIEYQKLNDIELEKILLGYKSNTPLGMLKEYTDFRISIAGAQEKTA
LLLKDNHWCVPHNNTPTTHIIKLPIGVIESHSYTMDLSNSVENEYLCTLIAKEFDLPVPHCSIIKTKNVKALAVERFDRK
YSSDKSWIIRLPQEDFCQILNIPSALKYENEGGPGIIDIMKYLLGSSNADQDRFHFMKAQVLFWLLAATDGHAKNFSVFI
NQEGRFHLTPLYDIMSIYPNFGGRGLNPRDAKLAMGLKASRGKKYAIEQIFPRHFYQTAEAVGFDPSLMSDILRYFYQEM
DDVIQRVKQQLPEDFPSEISNTILDGMKARAVRLANI

Nucleotide

• Length: 1314 bp

>T143462 NZ_CP061102:c47860-47579 [Escherichia coli]
ATGATGGAGATATTCTGGACCATGCTGGCCAGCCTGGACAGGAAGCGCATTCGTGAGAATATTGCGGAGCAGAACCTGAT
GGCTGCCATTGAACTGGACGAACGGATTGGTTATTCAGCCAGCAGCCTGGCCGGGCAGCCGTATAAAGGCCGCAACGGTC
GTGTGGAAGGTACCCGGGAACTGGTTATTCACCCGCATTTTGTTCTGGTTTATGAGGTCGACAGCCAGTGGGGAAAAGTG
TATATCCTGCGAGTATTGCATACTGCACAGAAGTGGCCATAG

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9878.43 Da
• Isoelectric Point: 10.0139

>AT143462 WP_004249771.1 NZ_CP046049:430533-430790 [Proteus mirabilis]
MIIINAYQLSVHLKDVRLTKKLSQSKVAQKVGIRQDTVSNFELNPNSTKLETFFKLLSALNLEMEIHPKDAKNNLAHKSE
WKEEW

Nucleotide

• Length: 258 bp

>AT143462 NZ_CP061102:c48126-47857 [Escherichia coli]
ATGGCACAGGTTAATATGAGTTTAAGAATCGACGCAGAACTGAAGGATGCTTTTATGGCCGCTGCAAAAAGCATGGACCG
TAATGGCTCTCAGTTAATCCGGGATTTTATGCGCCAGACCGTTGAACGGCAGCATAATACCTGGTTCCGCGACCAGGTTA
CGGCAGGACGTCAGCAACTCGAGCACGGTGATGTGCTTCCCCATGACATGGTCGAATCCTCTGCTGCAGCATGGCGGGAT
GAAATGAGCAGGAAGGTTGCCGGTAAATGA

Structures

Antitoxin

• AlphaFold DB: B4F0T9