Detailed information of TA system TA143460

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-MqsA
• Location: 3956252..3956951
• Replicon: chromosome
• Accession: NZ_CP046048
• Organism: Proteus mirabilis strain HN2p

Toxin (Protein)

• Gene name: relE
• Uniprot ID: B4EZ96
• Locus tag: GJR66_RS18305
• Protein ID: WP_004249093.1
• Coordinates: 3956252..3956638 (+)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: B4EZ97
• Locus tag: GJR66_RS18310
• Protein ID: WP_004246828.1
• Coordinates: 3956631..3956951 (+)
• Length: 107 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GJR66_RS18290	3952271..3952852	-	582	WP_004246833.1	DNA-3-methyladenine glycosylase I	-
GJR66_RS18295	3953103..3954008	+	906	WP_004246832.1	glycine--tRNA ligase subunit alpha	-
GJR66_RS18300	3954018..3956090	+	2073	WP_041701373.1	glycine--tRNA ligase subunit beta	-
GJR66_RS18305	3956252..3956638	+	387	WP_004249093.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GJR66_RS18310	3956631..3956951	+	321	WP_004246828.1	helix-turn-helix domain-containing protein	Antitoxin
GJR66_RS18315	3957000..3957434	-	435	WP_004246827.1	hypothetical protein	-
GJR66_RS18320	3957427..3958311	-	885	WP_110706881.1	endonuclease/exonuclease/phosphatase family protein	-
GJR66_RS18325	3958528..3959814	-	1287	WP_049256516.1	DUF3748 domain-containing protein	-
GJR66_RS18330	3959951..3960568	+	618	WP_004246822.1	trimeric intracellular cation channel family protein	-
GJR66_RS18335	3960870..3961493	+	624	WP_004246821.1	guanylate kinase	-
GJR66_RS18340	3961548..3961823	+	276	WP_004246820.1	DNA-directed RNA polymerase subunit omega	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (1-125)

Antitoxin

• Cro/C1-type helix-turn-helix domain (53-95)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14294.64 Da
• Isoelectric Point: 10.0642

>T143460 WP_004249093.1 NZ_CP046048:3956252-3956638 [Proteus mirabilis]
MVIYKTKMFKNSFKKITLTNAELIAIATEVLAGQFEADLGGGVIKKRACIQGKGKSSGIRTIIFYKQGNNLFFADGWKKS
SLSSKKTKEITDDELESYKDLAKDLFNANQNKIDKMIALGLLTEVKYD

Nucleotide

• Length: 387 bp

>T143460 NZ_CP061101:c4249037-4248864 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 107 a.a.
• Molecular weight: 12126.21 Da
• Isoelectric Point: 10.4411

>AT143460 WP_004246828.1 NZ_CP046048:3956631-3956951 [Proteus mirabilis]
MTDKMKEMLKFAQEMEDAGVTDGSTTKRIAARIKAKELQAKYKHPTPMTGEDIKRIREQNNISQSSLALILNMTKESVSK
WERNEIKPNGAALRLLHLIDKKILFI

Nucleotide

• Length: 321 bp

>AT143460 NZ_CP061101:c4249361-4249065 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CTTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Structures

Toxin

• AlphaFold DB: B4EZ96

Antitoxin

• AlphaFold DB: B4EZ97