Detailed information of TA system TA143459

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): VapXD/-
• Location: 3626468..3626938
• Replicon: chromosome
• Accession: NZ_CP046048
• Organism: Proteus mirabilis strain HN2p

Toxin (Protein)

• Gene name: VapD
• Uniprot ID: A0A8E4FU70
• Locus tag: GJR66_RS16800
• Protein ID: WP_060555119.1
• Coordinates: 3626660..3626938 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: VapX
• Uniprot ID: A0A8E4FTC4
• Locus tag: GJR66_RS16795
• Protein ID: WP_060555126.1
• Coordinates: 3626468..3626656 (+)
• Length: 63 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GJR66_RS16760	3621776..3622975	-	1200	WP_049218680.1	lipoprotein-releasing ABC transporter permease subunit LolC	-
GJR66_RS16765	3623277..3623678	+	402	WP_049218678.1	PPC domain-containing protein	-
GJR66_RS16770	3624095..3624496	-	402	WP_060555124.1	phage holin family protein	-
GJR66_RS16775	3624578..3624931	-	354	WP_060555123.1	hypothetical protein	-
GJR66_RS16780	3625003..3625251	-	249	WP_060555122.1	DUF883 family protein	-
GJR66_RS16785	3625701..3626048	+	348	WP_060555121.1	type II toxin-antitoxin system RelE/ParE family toxin	-
GJR66_RS16790	3626048..3626347	+	300	WP_060555120.1	XRE family transcriptional regulator	-
GJR66_RS16795	3626468..3626656	+	189	WP_060555126.1	DUF5397 family protein	Antitoxin
GJR66_RS16800	3626660..3626938	+	279	WP_060555119.1	virulence factor	Toxin
GJR66_RS16805	3627405..3628895	+	1491	WP_060555118.1	YadA-like family protein	-
GJR66_RS16810	3629082..3629156	-	75	Protein_3258	IS200/IS605 family transposase	-
GJR66_RS16815	3629150..3629257	-	108	Protein_3259	transposase	-
GJR66_RS16820	3629307..3629513	-	207	WP_060555117.1	hypothetical protein	-
GJR66_RS16825	3629510..3630133	-	624	WP_060555116.1	ParA family protein	-
GJR66_RS16830	3630602..3631120	-	519	WP_060555115.1	ClbS/DfsB family four-helix bundle protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	tet(J) / blaCMY-2	-	3483515..3670314	186799

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Virulence-associated protein D / CRISPR associated protein Cas2 (1-86)

Antitoxin

• Protein of unknown function DUF5397 (5-62)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10589.85 Da
• Isoelectric Point: 6.4606

>T143459 WP_060555119.1 NZ_CP046048:3626660-3626938 [Proteus mirabilis]
MYAISFDLTVADTEKHHPKGVSQAYNDISSTLKRYGFERIQGSLYTNSDENMANLFQAMNALKALEWFPNSVRDIRAFRI
EQWSDFTSSMKG

Nucleotide

• Length: 279 bp

>T143459 NZ_CP061101:c4060821-4060447 [Escherichia coli]
ATGAAAACATTACCCGACACGCATGTACGGGAGGTATCGTGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGGTTACTGGACCAGCATTACAGCCTTACGCTGAATGACACACCGTTCGTGGATGAACGCGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTGAACTTTCTCGTGGAAAAATACGCACTGGTACGAACTGACCAGCCGGGATTC
AGCGCAGGAGCCTCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCCGGGCAACAGGCCTGATGACCCGCCA
CAACTACAGAACGGTGAACAATATCACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 63 a.a.
• Molecular weight: 6787.78 Da
• Isoelectric Point: 3.9020

>AT143459 WP_060555126.1 NZ_CP046048:3626468-3626656 [Proteus mirabilis]
MNTVQIPTGSIKTFGLFGIPYEVGEPAQELPDGDILVNIILVQTGEKELYKLSKLLQDPDAE

Nucleotide

• Length: 189 bp

>AT143459 NZ_CP061101:c4061279-4060911 [Escherichia coli]
GTGTCAGACACACTTTCCGGGACAACGCATCCCGATGACAATGACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGAGATGCGGATGCGTACCCTCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGAAATTAATGCTCACCAGTGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCAGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCTGAAACGAAAAAGTAA