Detailed information of TA system TA143450

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/PRK09812-MazE
• Location: 1393102..1393685
• Replicon: chromosome
• Accession: NZ_CP046045
• Organism: Acinetobacter towneri strain 19110F47

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: -
• Locus tag: GJD93_RS07730
• Protein ID: WP_096900853.1
• Coordinates: 1393350..1393685 (+)
• Length: 112 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: N9CN57
• Locus tag: GJD93_RS07725
• Protein ID: WP_004973383.1
• Coordinates: 1393102..1393350 (+)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GJD93_RS07705	1388305..1389192	-	888	WP_004973373.1	sulfite exporter TauE/SafE family protein	-
GJD93_RS07710	1389419..1390594	+	1176	WP_004973375.1	lipid-A-disaccharide synthase	-
GJD93_RS07715	1390600..1391553	-	954	WP_096900855.1	AraC family transcriptional regulator	-
GJD93_RS07720	1391720..1392925	+	1206	WP_004973378.1	alkane 1-monooxygenase	-
GJD93_RS07725	1393102..1393350	+	249	WP_004973383.1	hypothetical protein	Antitoxin
GJD93_RS07730	1393350..1393685	+	336	WP_096900853.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
GJD93_RS07735	1393775..1394974	-	1200	WP_154320715.1	acyl-CoA dehydrogenase family protein	-
GJD93_RS07740	1395026..1396336	-	1311	WP_154320716.1	acyl-CoA dehydrogenase family protein	-
GJD93_RS07745	1396606..1397979	-	1374	WP_004973390.1	AarF/ABC1/UbiB kinase family protein	-
GJD93_RS07750	1398123..1398428	-	306	WP_154320717.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-101)

Antitoxin

• Antitoxin PemI-like (3-80)

Sequences

Toxin

Protein

• Length: 112 a.a.
• Molecular weight: 12262.07 Da
• Isoelectric Point: 6.2162

>T143450 WP_096900853.1 NZ_CP046045:1393350-1393685 [Acinetobacter towneri]
MNQGDIYLISLDPTLGHEQQGKRPVFVISPKSFNLITKMPIVLPITSGGNFARNMGFAVELIGGKTTGIVRCDQSRALDF
EARHARFLESSHADVLDEVLARYIAIFSPED

Nucleotide

• Length: 336 bp

>T143450 NZ_CP061101:2022468-2022571 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 8866.38 Da
• Isoelectric Point: 7.3181

>AT143450 WP_004973383.1 NZ_CP046045:1393102-1393350 [Acinetobacter towneri]
MIHTNLRKVGGSVMLAVPPTLLKSLGLDAGKEVSLSIVNGKLVVEPKAKPSYKLADLLQEHQALALEQDAWHDVKAVGRE
EI

Nucleotide

• Length: 249 bp

>AT143450 NZ_CP061101:c2022609-2022338 [Escherichia coli]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTGAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Structures

Antitoxin

• AlphaFold DB: N9CN57