Detailed information of TA system TA143445

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 181895..182483
• Replicon: chromosome
• Accession: NZ_CP046045
• Organism: Acinetobacter towneri strain 19110F47

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: -
• Locus tag: GJD93_RS01825
• Protein ID: WP_154320208.1
• Coordinates: 182196..182483 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: -
• Locus tag: GJD93_RS01820
• Protein ID: WP_154320207.1
• Coordinates: 181895..182209 (-)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GJD93_RS01795	178536..178871	-	336	WP_000618087.1	IS66 family insertion sequence element accessory protein TnpB	-
GJD93_RS01800	178868..179251	-	384	WP_004828545.1	IS66 family insertion sequence hypothetical protein	-
GJD93_RS01805	179906..180694	-	789	WP_154320109.1	hypothetical protein	-
GJD93_RS01810	180661..181134	-	474	WP_154320110.1	PD-(D/E)XK nuclease family protein	-
GJD93_RS01815	181204..181767	+	564	WP_154320111.1	macro domain-containing protein	-
GJD93_RS01820	181895..182209	-	315	WP_154320207.1	BrnA antitoxin family protein	Antitoxin
GJD93_RS01825	182196..182483	-	288	WP_154320208.1	BrnT family toxin	Toxin
GJD93_RS01830	182950..183450	-	501	WP_074163995.1	META domain-containing protein	-
GJD93_RS01835	183453..184136	-	684	WP_074163994.1	hypothetical protein	-
GJD93_RS01840	184462..185166	-	705	WP_001067784.1	IS6-like element IS1006 family transposase	-
GJD93_RS01845	185230..186060	+	831	Protein_172	IS91-like element ISVsa3 family transposase	-
GJD93_RS01850	186091..186975	+	885	WP_001447541.1	DUF3363 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (41-96)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11263.65 Da
• Isoelectric Point: 5.2134

>T143445 WP_154320208.1 NZ_CP046045:c182483-182196 [Acinetobacter towneri]
MEQYFEWDEAKNRKNQKKHDVSFETASLVFDDPLRISIQDRHTDGEERWQTIGKVKGVLMLLIAHTIFDEDDCEIIRIIS
ARQVTKAERDKYEHG

Nucleotide

• Length: 288 bp

>T143445 NZ_CP061101:1026319-1026654 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTATACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 12179.78 Da
• Isoelectric Point: 8.4842

>AT143445 WP_154320207.1 NZ_CP046045:c182209-181895 [Acinetobacter towneri]
MSTVRYSRKELNAKFSEKQDAEIERLLAKGTVPDDQLDLSDIPEITDWSNAVRHNQFYRPVKQQTSIRLDADVLAWFKAQ
GKGYQTRMNEILRDAMLKELKNHQ

Nucleotide

• Length: 315 bp

>AT143445 NZ_CP061101:1026071-1026319 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGAGATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGTCGAAAGATAAGGAA
GTCTGGTAA