Detailed information of TA system TA143444

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/upstrm_HI1419-dnstrm_HI1420
• Location: 119976..120564
• Replicon: plasmid p19110F47-2
• Accession: NZ_CP046044
• Organism: Acinetobacter towneri strain 19110F47

Toxin (Protein)

• Gene name: relE
• Uniprot ID: N9CKJ9
• Locus tag: GJD93_RS00815
• Protein ID: WP_004647749.1
• Coordinates: 120268..120564 (-)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: GJD93_RS00810
• Protein ID: WP_004969304.1
• Coordinates: 119976..120266 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GJD93_RS00780	115545..116141	+	597	WP_074163998.1	LysE family transporter	-
GJD93_RS00785	116169..116987	-	819	WP_032073151.1	AraC family transcriptional regulator	-
GJD93_RS00790	117118..117399	-	282	WP_000985610.1	putative addiction module antidote protein	-
GJD93_RS00795	117400..117702	-	303	WP_154320144.1	type II toxin-antitoxin system RelE/ParE family toxin	-
GJD93_RS00800	117952..118161	+	210	WP_004976362.1	hypothetical protein	-
GJD93_RS00805	118411..119865	+	1455	WP_026438532.1	SulP family inorganic anion transporter	-
GJD93_RS00810	119976..120266	-	291	WP_004969304.1	putative addiction module antidote protein	Antitoxin
GJD93_RS00815	120268..120564	-	297	WP_004647749.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GJD93_RS00820	121000..122082	-	1083	WP_004969299.1	hypothetical protein	-
GJD93_RS00825	122556..123122	+	567	WP_006581699.1	recombinase family protein	-
GJD93_RS00830	123305..124538	+	1234	Protein_117	IS3 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	mph(E) / msr(E) / erm(B) / blaOXA-58 / floR / tet(M) / aph(3')-Ia / aac(3)-IVa / aph(4)-Ia / sul2	-	1..143035	143035

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-95)

Antitoxin

• Cro/C1-type helix-turn-helix domain (47-92)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11584.25 Da
• Isoelectric Point: 5.8801

>T143444 WP_004647749.1 NZ_CP046044:c120564-120268 [Acinetobacter towneri]
MIEIKRLPEFDEWLDGIKDNMTRIRLNRRLDKVQRGNWGDIKPLQDGVWEMREFFGSGWRMYYIQHGDVVIVMLGGGDKS
TQQQDIDRAVKLSKTLED

Nucleotide

• Length: 297 bp

>T143444 NZ_CP061101:891972-892379 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGTGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10524.19 Da
• Isoelectric Point: 5.1133

>AT143444 WP_004969304.1 NZ_CP046044:c120266-119976 [Acinetobacter towneri]
MVKVADLPSFDMAESLKTEEDIVMYLNMVLEENDPAELAHALGVIAKARGMTQIAKEAGIGREALYKALRQDSAPRFDTI
NRVVNALGLKLTVQHA

Nucleotide

• Length: 291 bp

>AT143444 NZ_CP061101:891725-891991 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA