Detailed information of TA system TA143442

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 15534..16122
• Replicon: plasmid p19110F47-1
• Accession: NZ_CP046043
• Organism: Acinetobacter towneri strain 19110F47

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: -
• Locus tag: GJD93_RS00085
• Protein ID: WP_086384391.1
• Coordinates: 15835..16122 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: -
• Locus tag: GJD93_RS00080
• Protein ID: WP_023274838.1
• Coordinates: 15534..15848 (-)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GJD93_RS00045	10894..11268	+	375	WP_004676020.1	hypothetical protein	-
GJD93_RS00050	11386..11667	+	282	WP_154320108.1	type II toxin-antitoxin system RelE/ParE family toxin	-
GJD93_RS00055	11660..11932	+	273	WP_004676016.1	helix-turn-helix domain-containing protein	-
GJD93_RS00060	12017..13342	-	1326	Protein_11	ISNCY family transposase	-
GJD93_RS00065	13545..14333	-	789	WP_154320109.1	hypothetical protein	-
GJD93_RS00070	14300..14773	-	474	WP_154320110.1	PD-(D/E)XK nuclease family protein	-
GJD93_RS00075	14843..15406	+	564	WP_154320111.1	macro domain-containing protein	-
GJD93_RS00080	15534..15848	-	315	WP_023274838.1	BrnA antitoxin family protein	Antitoxin
GJD93_RS00085	15835..16122	-	288	WP_086384391.1	BrnT family toxin	Toxin
GJD93_RS00090	16610..16939	+	330	WP_016658833.1	four-helix bundle copper-binding protein	-
GJD93_RS00095	17067..17359	-	293	Protein_18	type II toxin-antitoxin system RelE/ParE family toxin	-
GJD93_RS00100	17349..17594	-	246	WP_000246755.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	-
GJD93_RS00105	17774..18718	-	945	WP_154320112.1	copper resistance protein B	-
GJD93_RS00110	18705..19043	-	339	WP_004897649.1	hypothetical protein	-
GJD93_RS00115	19030..20841	-	1812	WP_154320113.1	copper resistance system multicopper oxidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	blaNDM-1 / aph(3')-VI / aac(3)-IId	-	1..47094	47094

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (41-96)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11295.67 Da
• Isoelectric Point: 5.2134

>T143442 WP_086384391.1 NZ_CP046043:c16122-15835 [Acinetobacter towneri]
MEQYFEWDEAKNRKNQKKHDVSFETASLVFDDPLRISIQDRHTDGEERWQTIGKVKGVLMMLVAHTIFDEDDCEIIRIIS
ARQVTKAERDRYEHG

Nucleotide

• Length: 288 bp

>T143442 NZ_CP061101:c621087-620623 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTTTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAAGCCTCACCCAAGAGACAGAAGAAAACCATTGA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 12298.98 Da
• Isoelectric Point: 9.4860

>AT143442 WP_023274838.1 NZ_CP046043:c15848-15534 [Acinetobacter towneri]
MSMVRYSRKELNEKFSDKQDAEIQRLLAKGTVPDDQLDLSDMPEITDWSNAVRHNRFYRPVKQQTSIRLDADVLAWFKAQ
GKGYQTRMNEILRDAMLKELKNHQ

Nucleotide

• Length: 315 bp

>AT143442 NZ_CP061101:c621422-621087 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTCTTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA