Detailed information of TA system TA143437

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PfiT-PfiA/ParE-Phd
• Location: 21760..22371
• Replicon: plasmid pPor1_6
• Accession: NZ_CP046036
• Organism: Pseudomonas coronafaciens pv. oryzae str. 1_6

Toxin (Protein)

• Gene name: PfiT
• Uniprot ID: A0A3M3D151
• Locus tag: POR16_RS25940
• Protein ID: WP_003436792.1
• Coordinates: 22021..22371 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: PfiA
• Uniprot ID: A0A3M6EYM7
• Locus tag: POR16_RS25935
• Protein ID: WP_003436790.1
• Coordinates: 21760..22008 (+)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
POR16_RS25910	17093..18004	+	912	WP_003436770.1	alpha/beta hydrolase	-
POR16_RS25915	18188..18646	-	459	WP_032610010.1	helix-turn-helix transcriptional regulator	-
POR16_RS25920	18743..19690	+	948	WP_003436783.1	aromatic alcohol reductase	-
POR16_RS25925	20102..20365	+	264	Protein_21	Mov34/MPN/PAD-1 family protein	-
POR16_RS25930	20466..21428	-	963	WP_003436787.1	tyrosine-type recombinase/integrase	-
POR16_RS25935	21760..22008	+	249	WP_003436790.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
POR16_RS25940	22021..22371	+	351	WP_003436792.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
POR16_RS25945	22474..23247	-	774	WP_003436798.1	LuxR family transcriptional regulator	-
POR16_RS25950	23356..24456	+	1101	WP_003436801.1	polyamine ABC transporter substrate-binding protein	-
POR16_RS25955	24555..24752	+	198	WP_003436803.1	hypothetical protein	-
POR16_RS25960	24764..25159	-	396	WP_003436805.1	helix-turn-helix domain-containing protein	-
POR16_RS25965	25374..25886	+	513	WP_003436807.1	hypothetical protein	-
POR16_RS25970	25975..26217	+	243	WP_003436809.1	hypothetical protein	-
POR16_RS25975	26230..26937	+	708	WP_003436811.1	lytic transglycosylase domain-containing protein	-
POR16_RS25980	26937..27272	+	336	WP_003407111.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..51521	51521

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-101)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13002.85 Da
• Isoelectric Point: 4.2080

>T143437 WP_003436792.1 NZ_CP046036:22021-22371 [Pseudomonas coronafaciens pv. oryzae str. 1_6]
MNTFALRFTEVAQQSIEDQVEHLAITQGFSSAAQRIDTLIDAIQDKLLSTPLGYPVSPQLSELGVLHYRELNTDGYRIFY
EVMQSVDIDEIVISLVLGGKQSVEQALIRYCLLQAI

Nucleotide

• Length: 351 bp

>T143437 NZ_CP061088:c327225-326935 [Brucella sp. 6810]
ATGATCGAAGTTCGCCAAACCACAGCATTCACAAAATGGCTCAACGAGCTTCGAGACACCAATGCGCGTTTAAGGATTGC
CACTCGTATCCGACGAATGGAGCTTGGCAATCCAGGTGATGTGAAAGCCGTTGGTGAAGGTGTGAGCGAGATGCGTATTA
CCTATGGTCAAGGCTATCGGGTTTATTTCGTGACCATGGGTTCAACCATTGTCGTTCTGTTATGTGGCGGTGACAAGTCA
TCACAAAGCCGGGATATTGCCGTGGCGAAACAAATGGCGAAGGAAATTTAG

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9361.71 Da
• Isoelectric Point: 9.8452

>AT143437 WP_003436790.1 NZ_CP046036:21760-22008 [Pseudomonas coronafaciens pv. oryzae str. 1_6]
MRVETISYLKRNAADLPLDEPLVVTQNGVPAYVVESYADRKRRDESIALVKLLAISSREYSQGKHCSTDELKARLSRRFA
HK

Nucleotide

• Length: 249 bp

>AT143437 NZ_CP061088:c326933-326637 [Brucella sp. 6810]
ATGGCCCTTGAAACCACTCGTTTCGATATTCTCGACCATCTCAAGACGCCGGAGGAGCGCCTTGCTTATCTCGAAGCAGC
ATTTGAGGATGGCGATCCTTCTCTTATCGCTCATGCCCTCGGCGATGTGGCCCGCTCCGTTGGGATGACTGCCGTTGCCA
AAGAAGCCGGCATTACCCGTGAGGCGCTTTATAGATCGCTCAGCGAAAACGGCGACCCGAAACTATCGACCTTGCTTGGC
GTTACCAAAGCCCTCGGCATTCATTTGACAGTCGGGCCAGACAAACACGCCGCCTGA

Structures

Toxin

• AlphaFold DB: A0A3M3D151

Antitoxin

• AlphaFold DB: A0A3M6EYM7