Detailed information of TA system TA143432

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 778511..779035
• Replicon: chromosome
• Accession: NZ_CP046028
• Organism: Staphylococcus chromogenes strain 1401

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: GJU84_RS03630
• Protein ID: WP_103159488.1
• Coordinates: 778682..779035 (+)
• Length: 118 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A0A3A0K2V6
• Locus tag: GJU84_RS03625
• Protein ID: WP_037577177.1
• Coordinates: 778511..778681 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GJU84_RS03600	774359..774832	+	474	WP_145400619.1	PH domain-containing protein	-
GJU84_RS03605	774825..776303	+	1479	WP_145400620.1	PH domain-containing protein	-
GJU84_RS03610	776300..776815	+	516	WP_165885554.1	PH domain-containing protein	-
GJU84_RS03615	776812..777162	+	351	WP_145400622.1	holo-ACP synthase	-
GJU84_RS03620	777278..778426	+	1149	WP_165885555.1	alanine racemase	-
GJU84_RS03625	778511..778681	+	171	WP_037577177.1	hypothetical protein	Antitoxin
GJU84_RS03630	778682..779035	+	354	WP_103159488.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
GJU84_RS03635	779099..780103	+	1005	WP_145400623.1	PP2C family protein-serine/threonine phosphatase	-
GJU84_RS03640	780184..780510	+	327	WP_145400624.1	anti-sigma factor antagonist	-
GJU84_RS03645	780513..780992	+	480	WP_037577169.1	anti-sigma B factor RsbW	-
GJU84_RS03650	780967..781737	+	771	WP_037577167.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (2-108)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 118 a.a.
• Molecular weight: 13064.34 Da
• Isoelectric Point: 10.5127

>T143432 WP_103159488.1 NZ_CP046028:778682-779035 [Staphylococcus chromogenes]
MKRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIDKKKYKLDKDSVILLEQIRT
VDKKRLKEKLTFLSDKKMKEVNAALGISLGLHMNQHK

Nucleotide

• Length: 354 bp

>T143432 NZ_CP061087:c1280559-1279813 [Bacillus velezensis]
ATGCTTTTGTTTTTTCAGATCATGGTGTGGACGATGGCGGCCGCACTCATTTTATATGTGTACGCATCCTGGCGGTATGA
GGCAAAGGTAAAAGAAAAAATGTTTGCCATCCGTAAAACGTGGTATTTACTATTTGTACTGGGCTCCATGGTCTATTGGA
CATATGATCCCGAATCGCTCTTTGCCGCATGGAGACAATATTTGATTGTTGCCGTCTGCTTTGCCCTTATTGATGCGTTT
ATCTTTTTGAGCGCGTATATCAAAAAGCTCGCCGGCAATGAATTAGAGACGGATACGCGGGAAATTTTAGAAGAAAATAA
CGAAATGCTTCACTCCTATCTTGAAAAACTGAAAACCTATCAATATCTGTTGAAAAATGAACCGATCCATGTATATTATG
GAAGTACAGAAGCTTATGCGGAAGGCATCACCAGACTGCTGGCCGCTTATGCCGAAAAAATGAATGTCACGGCTTCCTTG
TGCGACTATTCCGCACAATCGGATAAGGACCGCTTGACGGAGCATATGTCTGATGCGGCGGACGTTCAGTCCCGTCTTAA
CCGTAAAGACGTCTACTATGATCAAAAAGGCAGGCTTGTGCTTATTCCGTTTACAGTCCAGAACCGCCATTACGTGATCA
AACTCACGTCGGAAAACCTGTTGACGGAATTCGATTATTTGCTTTTCACATCGCTAACGAGCATTTATGATTTAATGCTG
CCAATAGAAGAGGAAGGTGACGGTTAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6328.01 Da
• Isoelectric Point: 4.2313

>AT143432 WP_037577177.1 NZ_CP046028:778511-778681 [Staphylococcus chromogenes]
MSAFNQTRVKSLEQSLKEGYVQMADLNLSLATEAYFVECEACDCNESHLLSNHKDD

Nucleotide

• Length: 171 bp

>AT143432 NZ_CP061087:c1279812-1279642 [Bacillus velezensis]
ATGGAGCAGGCATTTCAAAACGGGCAGCCGGAACAAAGAGCGAAACCGTTCAAAATGTTTAAAAAACGTTCAATGACAAG
TATCGCAAGTTACCAGGTCAGCCCTCATACCGCCAGAATTTTCAAAGAAAACGAGCGGCTGATTGACAAGTATAAACAAA
AAAAAGCTTGA

Structures

Antitoxin

• AlphaFold DB: A0A3A0K2V6