Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 61062..61639 Replicon plasmid pT82A
Accession NZ_CP046026
Organism Escherichia coli strain C439

Toxin (Protein)


Gene name mazF Uniprot ID U5N651
Locus tag GJH08_RS22905 Protein ID WP_023159957.1
Coordinates 61307..61639 (+) Length 111 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID U5N340
Locus tag GJH08_RS22900 Protein ID WP_023159984.1
Coordinates 61062..61307 (+) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GJH08_RS22865 56207..56533 + 327 WP_000497519.1 tyrosine-type recombinase/integrase -
GJH08_RS22870 56721..56960 - 240 WP_001353740.1 DUF1819 family protein -
GJH08_RS22875 57533..58012 + 480 WP_048821772.1 Hcp family type VI secretion system effector -
GJH08_RS22880 58021..58599 + 579 WP_048822182.1 hypothetical protein -
GJH08_RS22885 59360..59728 + 369 WP_048821769.1 hypothetical protein -
GJH08_RS22890 59826..60053 - 228 WP_048821767.1 hypothetical protein -
GJH08_RS22895 60162..60785 - 624 WP_023159958.1 AAA family ATPase -
GJH08_RS22900 61062..61307 + 246 WP_023159984.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
GJH08_RS22905 61307..61639 + 333 WP_023159957.1 endoribonuclease MazF Toxin
GJH08_RS22910 61670..62050 + 381 WP_048821763.1 hypothetical protein -
GJH08_RS22915 62139..62279 - 141 WP_072022937.1 type I toxin-antitoxin system Hok family toxin -
GJH08_RS22920 62599..63057 - 459 WP_048821762.1 hypothetical protein -
GJH08_RS22925 63449..63718 - 270 WP_048821758.1 hypothetical protein -
GJH08_RS22930 64013..64966 + 954 WP_023159956.1 site-specific integrase -
GJH08_RS22935 64980..65798 + 819 WP_023159955.1 DUF4365 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid lnu(G) / aadA14 / blaOXA-1 / aac(6')-Ib-cr / blaCTX-M-15 / floR / aph(6)-Id / aph(3'')-Ib / sul2 / dfrA1 - 1..104812 104812


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(9-107)

Antitoxin

SpoVT-AbrB domain

(7-49)


Sequences

Toxin        


Download         Length: 111 a.a.        Molecular weight: 12097.88 Da        Isoelectric Point: 7.0813

>T143426 WP_023159957.1 NZ_CP046026:61307-61639 [Escherichia coli]
MVSRYVPDSGDLIWIDFDPVEGHEQGGHRPAVVLSPFAYNNKVGLLLCVPCTTKGKGYPFECELSNERDSYALADQVTCV
DWRARKVTKKGKVEASELAEIKAKAKALIG

Download         Length: 333 bp

>T143426 NZ_CP061083:2103492-2104817 [Pantoea sp. MT58]
ATGAGCCGGAGACAGCAGCGTTTAGTGATATGGATGAACGGTATTCAGGTTGGATTCTGGAACAAAAGCAAAGGAGAAGA
CACGCTGCAATATCTTCCTGAATGGAGTGCCGACGCGCAAGGAAGACCGTTATCGCTCTCTTTGCCTTTTACCCCCGGCG
ATCAAATCTGGCGCGGAAATGTGGTACGTGACTACTTTGATAATTTATTACCGGATAGTGAAGGGATTCGCAGGCGTCTG
GCAATGCGGTATCAGGCAGAAAGTCTCGACCCTTTTGACCTGTTGACCGAGTTGGGAAAAGATTGCGTAGGCGCGATACA
GTTACTGAAAGAGGGCGATGAACCCACGGATTTATATTGCGTAAAATATCGGCCGCTCTCTGAATCAGAGATCGCAATGA
CATTGCGAAATACCACGGCGGCATCATTACCCGGCAGACAGAGTGATACAGGCGACTTACGGCTATCCATTGCCGGTGCG
CAGGAAAAAACAGCGTTATTATGGCATGACGATCGGTGGTGCAGGCCTGAAGGGAATACGCCAACGACGCATATTTTCAA
GTTGCCGCTTGGCCTGGTCGGCAATATGAAAGCTGATATGAGTACATCCGTGGAAAACGAATGGCTCTGTTCGCTGCTAC
TTGAACAATTCGGCATCCCGGTCGCAAGAACAGAAATCGCTCAATTCGAAGATCAGAAAGTCCTGGTGGTTGAACGTTTT
GACCGCAAATGGTCAGGTGATCAGCGTTGGATCATTCGTTTACCTCAGGAAGATATGTGTCAGGCTGCGGGGATGTCACC
ATTACGCAAATATCAGGCCGACGGCGGGCCAGGTATCTCTGAAATTATGGAGATTCTGAGTCATTCAGATCACGCAGAAC
AGGACAGATTGCATTTCTTTAAAGCACAGATAATTTTCTGGGTACTGGCGGCCACAGATGGCCATGCTAAAAATTTCAGT
ATTGCGATAGGGCCTCAGGGGCATTATTGCCTTACGCCACTTTATGATGTTTTATCGGCATGGTCGGTCATCGGAAATGG
CAATAATCAGATAGCCTGGCAAAAATGCAAACTGGCGATGGCCGCTCGCGGCAGCAGCAACTATTACAAACTCTACAGAA
TACAAAGACGGCACTGGATTAACCACGGTGAGCAGACCGGCCTGAGTAAAAAGCAGGTTGAGATGATGATGGATGAAATA
ATTTCTGACGCACCGGACGTCATCGAGCGTGTTCGCGCTCAACTGCCAGATCAGTTCCCGTCAGAACTGGCAGAACGCGT
GTTTGATGGCATAACGCAGCAATGCAGGCGTCTGGCCGGAAATTAG

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 8786.20 Da        Isoelectric Point: 5.0351

>AT143426 WP_023159984.1 NZ_CP046026:61062-61307 [Escherichia coli]
MAQVTVKKWGNSPSVRLPVAIMQKAALSVDDTVEIAVEEGRIIITPVKAVEYSLDNLLAGITPENIHEKVDFGARTGKEL
I

Download         Length: 246 bp

>AT143426 NZ_CP061083:2103191-2103487 [Pantoea sp. MT58]
ATGAAAAATGTCTATCCGATAAAAACACTCAGCCAGTTGCGTCCTTTGCTTATTGGTTTTCGCAAAGCAAATGGACTGAC
GCAAAAAGATCTGTCTGAACGATTAGGCGTCACTCAGCAGACTTATGCGCGTCTGGAAGCCAACCCGGCCAGAGCCAGCA
TTGAACGCCTCTTCAAAATCTTCACACTGCTGGGCGTGGAGATGACTCTCTCTTCGGGAATAAGCTCATCCGCAGGAAAG
CAGACAGAAGAAAAGGGGACATTAGAAAATTCACCTGCGAGACGGGAGAAATGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P1BQ75

Antitoxin

Source ID Structure
AlphaFold DB A0A857E7M9

References