Detailed information of TA system TA143423

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yjhX-yjhQ/YjhX-GNAT
• Location: 4007756..4008570
• Replicon: chromosome
• Accession: NZ_CP046025
• Organism: Escherichia coli strain C439

Toxin (Protein)

• Gene name: yjhX
• Uniprot ID: S1PA82
• Locus tag: GJH08_RS19500
• Protein ID: WP_001054376.1
• Coordinates: 4007756..4008013 (+)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: yjhQ
• Uniprot ID: U9Z4B8
• Locus tag: GJH08_RS19505
• Protein ID: WP_001309181.1
• Coordinates: 4008025..4008570 (+)
• Length: 182 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GJH08_RS19480	4003044..4004150	+	1107	WP_001309184.1	N-acetylneuraminate epimerase	-
GJH08_RS19485	4004215..4005195	+	981	WP_000991438.1	9-O-acetyl-N-acetylneuraminic acid deacetylase	-
GJH08_RS19490	4005778..4007018	-	1241	Protein_3807	helicase YjhR	-
GJH08_RS19495	4007134..4007379	+	246	Protein_3808	GNAT family N-acetyltransferase	-
GJH08_RS19500	4007756..4008013	+	258	WP_001054376.1	hypothetical protein	Toxin
GJH08_RS19505	4008025..4008570	+	546	WP_001309181.1	N-acetyltransferase	Antitoxin
GJH08_RS19510	4008626..4009372	+	747	WP_000354251.1	class I SAM-dependent methyltransferase	-
GJH08_RS19515	4009541..4009759	+	219	Protein_3812	hypothetical protein	-
GJH08_RS19520	4009797..4009913	+	117	Protein_3813	VOC family protein	-
GJH08_RS19525	4010158..4011279	+	1122	WP_000010829.1	M42 family metallopeptidase	-
GJH08_RS19530	4011276..4011554	+	279	WP_000722973.1	PTS sugar transporter subunit IIB	-
GJH08_RS19535	4011566..4012879	+	1314	WP_000460843.1	galactitol-specific PTS transporter subunit IIC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	fimB	3999007..4016794	17787

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Putative toxin of bacterial toxin-antitoxin pair (1-85)

Antitoxin

• GNAT domain (18-121)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 9734.29 Da
• Isoelectric Point: 11.0090

>T143423 WP_001054376.1 NZ_CP046025:4007756-4008013 [Escherichia coli]
MNLSRQEQHTLHVLAKGRRIAHVRDSSGRVTSVECYSREGLLLTDCTLAVFKKLKTKKLIKSVNGQPYRINTTELNKVRA
QLDNR

Nucleotide

• Length: 258 bp

>T143423 NZ_CP061082:4787399-4787710 [Serratia liquefaciens]
ATGCATATAATTTCAAGGGAACCGTTCAACGAGGCCGCAATCCGCTACCCCAACGAAGCAGCCTCTCTCAACACAATCTA
CAAAACGCTGCGCCGGGGGACATTTCATACGCCGGAAGATTTAAAATCCCTTTTCCCCAGCCTGGACAGAATGAAGTACA
AAGCAAAATGGTGGGTCATTGATGTCGGAGGAAATCATCTCAGGATCTTATTCTTTGCCAGTTTTGATACCCAGAAAATA
TTTGTTAAACAGATAGTGACTCATGCAGAGTACGACAGATTACTGCAGCAGTACCGGAGGAACCCAAAATGA

Antitoxin

Protein

• Length: 182 a.a.
• Molecular weight: 19956.90 Da
• Isoelectric Point: 6.3277

>AT143423 WP_001309181.1 NZ_CP046025:4008025-4008570 [Escherichia coli]
MTVHHFTFHITDKSDASDIREVETRAFGFSKEADLVASLLEDESARPALSLLARYEGKAVGHILFTRATFKGEMDSPLMH
ILAPLAVIPEYQGMGVGGRLIRTGIEHLRLMGCQTVFVLGHATYYPRHGFEPCAGDKGYPAPYPIPEEHKACWMMQSLTA
QPMTLTGHIRCADPDETGALT

Nucleotide

• Length: 546 bp

>AT143423 NZ_CP061082:4787707-4788129 [Serratia liquefaciens]
ATGATCACCGATGCGCTTAAGGCCACCCATGAGCTGTTACGCGCCATACCCTTGTTAGGTGGCAGCACCTCGCCACAGGA
TTACCGTGATGCTCTGGCGTTAGTGGAACACCTGCTTGAAACCGACGAGCGCCACCCGCTGATTGACGTTCTGGCAGCCA
AGATCGTCGAGTATGAAGATAACAGCCCTGATTTTGCAGAGTTCAATCAGCGTATCGCACAGCTACCACAAGGTGTAGCC
GCGCTGAGCGTGCTGATGGAGCAATATGGCCTCAGTCAAAGCGACTTCGAAAACGAGATCGGCAAAAAGTCACTGGTGAG
CCAAATTCTAAGCGGAAAGCGATCGCTGACCATACCCCATATCATGGCGCTGGCAAAACGTTTCAACCTGCCGCCGGCCT
TGTTCTTGCCTGAAGCCCGTTAG

Structures

Toxin

• AlphaFold DB: S1PA82

Antitoxin

• AlphaFold DB: U9Z4B8