Detailed information of TA system TA143418

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yafW-ykfI/CbtA-CbeA
• Location: 3580338..3581017
• Replicon: chromosome
• Accession: NZ_CP046025
• Organism: Escherichia coli strain C439

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: P77692
• Locus tag: GJH08_RS17430
• Protein ID: WP_000854672.1
• Coordinates: 3580338..3580679 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yafW
• Uniprot ID: Q47684
• Locus tag: GJH08_RS17435
• Protein ID: WP_000070395.1
• Coordinates: 3580700..3581017 (-)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GJH08_RS17405	3575924..3576016	+	93	Protein_3401	sigma factor-binding protein Crl	-
GJH08_RS17410	3576055..3577110	-	1056	WP_000749863.1	phosphoporin PhoE	-
GJH08_RS17415	3577398..3578501	+	1104	WP_001285288.1	glutamate 5-kinase	-
GJH08_RS17420	3578513..3579766	+	1254	WP_000893278.1	glutamate-5-semialdehyde dehydrogenase	-
GJH08_RS17430	3580338..3580679	-	342	WP_000854672.1	type IV toxin-antitoxin system toxin YkfI	Toxin
GJH08_RS17435	3580700..3581017	-	318	WP_000070395.1	type IV toxin-antitoxin system antitoxin YafW	Antitoxin
GJH08_RS17440	3581036..3581257	-	222	WP_000691994.1	DUF987 domain-containing protein	-
GJH08_RS17445	3581266..3581742	-	477	WP_000811693.1	RadC family protein	-
GJH08_RS17450	3581758..3582216	-	459	WP_000211838.1	antirestriction protein	-
GJH08_RS17455	3582314..3582553	-	240	WP_000194654.1	DUF905 domain-containing protein	-
GJH08_RS17460	3582630..3583097	-	468	WP_001547765.1	hypothetical protein	-
GJH08_RS17465	3583120..3583563	-	444	WP_000824223.1	hypothetical protein	-
GJH08_RS17470	3583563..3583790	-	228	WP_001548158.1	hypothetical protein	-
GJH08_RS17475	3583786..3583977	-	192	Protein_3414	DeoR family transcriptional regulator	-
GJH08_RS17480	3584194..3585015	-	822	WP_000197389.1	DUF945 domain-containing protein	-
GJH08_RS17485	3585107..3585970	-	864	WP_001065553.1	GTPase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	3575218..3614031	38813

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (6-104)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12905.01 Da
• Isoelectric Point: 9.6543

>T143418 WP_000854672.1 NZ_CP046025:c3580679-3580338 [Escherichia coli]
MKTLPAITQRAVKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAADILRARQATGLLRQSRNNVVR

Nucleotide

• Length: 342 bp

>T143418 NZ_CP061082:1227724-1228092 [Serratia liquefaciens]
ATGCAGACTTTACCTGTGACCCCGAAACGGGTGGTCTACGCTTGCCCATCACCCGTTGTTGTCTGGCAATCCATGCTCAC
GTATCTGCTAGGTCAACATTACGGTTTGGCGCTTAACGATACCGAGTTCAGTGATGACACCGTTATCCATGAACACATTG
ACGCCGGGATCTCTCTGGCCGATGCATTGAATTTCACAGTGGAACAATTTGATTTGGTTCGCACCGATCGCAGGGGGTTC
TCCTGTCAGGAACAATCCCCGTTTATCACGGCTATCGATATTCTCCGTGCCCGTCGAGCTACCGGCCTGATGACCCGTAT
GGGTTACCAGACCATCACCTCGGTTATCCGGGGAGAAAAGCAGGCATGA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11939.59 Da
• Isoelectric Point: 6.7391

>AT143418 WP_000070395.1 NZ_CP046025:c3581017-3580700 [Escherichia coli]
MSNPTRGLQREITLRLGARLVQEGNRLHYLADRASITGKFSDIECRKLDETFPHFILQMESMLTTGELSPHHAHCVTLYH
NDLTCEADTLGSCGYVYIAIYPTQR

Nucleotide

• Length: 318 bp

>AT143418 NZ_CP061082:1227338-1227649 [Serratia liquefaciens]
ATGACATCAATCAATACCCCAGCCTGGGGACTCAAACGCAACGTCACTCCACAGTTTGGTGCCCGCCTGGTGCAGGAAGG
CAAACGACTACATTTTCTGGCCGACCGTGCCGATATCAGCGGGTCCTTCAGCGAAATACAGCTACGTGATGTAGATAACG
CCTTCCCGCAGTTCATCAACCACCTGGAACTGATGCTGCTTTCTGGTGAGCTCAATCCCAGTTATCAGCACTGCGTCACG
CGACTAACCTGTGAAGCCGATAGCCTGGGAGCACACGGCTATGTGTATCTCTCCATTTATCCTACTCCCTAA

Structures

Toxin

• AlphaFold DB: P77692

Antitoxin

• AlphaFold DB: Q47684