Detailed information of TA system TA143416

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2377422..2378060
• Replicon: chromosome
• Accession: NZ_CP046025
• Organism: Escherichia coli strain C439

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: GJH08_RS11445
• Protein ID: WP_000813794.1
• Coordinates: 2377884..2378060 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: GJH08_RS11440
• Protein ID: WP_001270286.1
• Coordinates: 2377422..2377838 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GJH08_RS11420	2372574..2373515	-	942	WP_001251304.1	ABC transporter permease	-
GJH08_RS11425	2373516..2374529	-	1014	WP_000220396.1	ABC transporter ATP-binding protein	-
GJH08_RS11430	2374547..2375692	-	1146	WP_000047424.1	ABC transporter substrate-binding protein	-
GJH08_RS11435	2375937..2377343	-	1407	WP_000760626.1	PLP-dependent aminotransferase family protein	-
GJH08_RS11440	2377422..2377838	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
GJH08_RS11445	2377884..2378060	-	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
GJH08_RS11450	2378282..2378512	+	231	WP_000494244.1	YncJ family protein	-
GJH08_RS11455	2378604..2380565	-	1962	WP_001303492.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
GJH08_RS11460	2380638..2381174	-	537	WP_000429155.1	DNA-binding transcriptional regulator SutR	-
GJH08_RS11465	2381227..2382441	+	1215	WP_001326689.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T143416 WP_000813794.1 NZ_CP046025:c2378060-2377884 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T143416 NZ_CP061082:450325-450609 [Serratia liquefaciens]
ATGCGTGTCGAATGGGATGAAGAAGCGCTACGGGACAGGGAACGTATTTTTGATTTTCTCTATCCTTTTAATCCACAGGT
TGCTGAGCAGGCTGATGCTGAGATTGATAAGGCAGTGAAGCGCCTGTTGGATTACCCTGAACTCGGTAAAATTTGGTACA
GACAGGCGCGTAAGTTATTGGTCAGCCAGGCTTCGTTATTAGTTTTATACGTTGTTGTCGACGACGTGATTAAGGTTTTA
GCCGTTGCTCATCAACGAGAGAAATTCCCCGACATAGAGAACTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT143416 WP_001270286.1 NZ_CP046025:c2377838-2377422 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT143416 NZ_CP061082:450029-450316 [Serratia liquefaciens]
ATGGAAAGCCGTATCCAGTTTCGCATTGAGGATGAGACTAAACGTTTGGCGCAGAAAGCCGCAGAAGCCAAGGGGATCAC
TTTGAGTGAGGCGTGTCGACGTTTGGCTGAGCAAATGGCGGATGAGCAACGGGCCACTGAGCAACATGAAAATTGGTTGA
AAGAAAAGGTTGATGCCGCTTTTGCCCGTTTGCATGAAGGAAGTGCGGTCTATCTTGACCAGCAACAGGTTGATGAAAGC
ATGGATGCCTTTAAGGCAAAAGTCCGAGCGAAATACGACCGAAAATAA

Structures

Toxin

• PDB: 6HPB