Detailed information of TA system TA143415

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 2294904..2296492
• Replicon: chromosome
• Accession: NZ_CP046025
• Organism: Escherichia coli strain C439

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: S1ELM3
• Locus tag: GJH08_RS11085
• Protein ID: WP_001125439.1
• Coordinates: 2295170..2296492 (+)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: GJH08_RS11080
• Protein ID: WP_001301023.1
• Coordinates: 2294904..2295170 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GJH08_RS11075	2290937..2294681	+	3745	Protein_2163	autotransporter barrel domain-containing lipoprotein	-
GJH08_RS11080	2294904..2295170	+	267	WP_001301023.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
GJH08_RS11085	2295170..2296492	+	1323	WP_001125439.1	type II toxin-antitoxin system serine/threonine protein kinase toxin HipA	Toxin
GJH08_RS11090	2296804..2297012	+	209	Protein_2166	helix-turn-helix transcriptional regulator	-
GJH08_RS11095	2297267..2298493	+	1227	Protein_2167	fimbrial biogenesis outer membrane usher protein	-
GJH08_RS11100	2298507..2299037	+	531	WP_000876763.1	type 1 fimbrial protein	-
GJH08_RS11105	2299050..2299553	+	504	WP_000825452.1	type 1 fimbrial protein	-
GJH08_RS11110	2299612..2300526	+	915	WP_000520676.1	fimbrial protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	2287924..2307713	19789

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (149-401)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-69)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49275.82 Da
• Isoelectric Point: 8.2482

>T143415 WP_001125439.1 NZ_CP046025:2295170-2296492 [Escherichia coli]
MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLASRYARPLSLSLPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYH
AKSRQPFDLLSEIGRDSVGAVTLIPEDETVTHPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVPDAEIIKAGNVRALAVERFDRRW
NAERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIARIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQ
AGGSYRLTPFYDIISAFPVLGGTGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLRFPEVQMHEILSDFARMIP
AALDNVKTSLPTDFPENVVTAVESNVLRLHGRLSREYGSK

Nucleotide

• Length: 1323 bp

>T143415 NZ_CP061081:4416758-4417180 [Marinomonas arctica]
ATGAACGACTCGATTCTTAATAAGCTAGCCACCATTGATCGCTGCCTAGTTCGTATTCGTGACGTTTACGCTTTAGCAAA
AGAAAACTTCGACACCGACTACACCAGCCAAGATTCGATCATTCTGAATCTACAGCGCGCGTGCGAAGCCAGCATTGATG
CCGCCAATATACTCAACAAACAACACCAGACTGGCATCCCACAGAGTAGCCGCGACAGCTTTGAACTGCTTAAAAAAGCA
GGCTTTATCCCTGCTCCACTAGCCTTAAACTTGCAAAAGATGGTCGGTCTTCGCAATATCGCGGTTCACGACTATCAGAC
CCTTAACTTAGAAATAGTAAAGCATGTTGTAGAGCATAGGCTTAGTGATTTCGAAGAGTTTGTTAAAGCAATGAAAAGCC
TTAATCTATCCAATTTAGTATAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10016.38 Da
• Isoelectric Point: 6.2267

>AT143415 WP_001301023.1 NZ_CP046025:2294904-2295170 [Escherichia coli]
MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISNFENNPDNTTLTTFFKILQSLELSMTLCDAKNASPES
TEQQNLEW

Nucleotide

• Length: 267 bp

>AT143415 NZ_CP061081:4416384-4416761 [Marinomonas arctica]
ATGCTCGATAGAAACAAGCTAATTCAAGCCGTAAAACGCCTGATGCCAAGGGCTAGATTGATTTACTTGTTTGGTTCTCA
AGCTGATGGTAGTGCTACGGCTTCCAGTGATATTGATATTGCCGTGCTACTGGATAAAAAAATGGACTCAGTAGCACGCT
TTGACCTCCAAGAAACACTCGCTATCGAGCTCAATCAAGATGTCGATTTGATAGATTTACTAGCAGCTTCAACCGTCTTA
CAAAACCAAGTTATCATGAATGGCGAGCTACTTTTTGGAAGTGAAAACGAACAAGCTAAGTTTGAAATGCAAATCATGTC
TATGTATCAACACTTGAACGAAGAGCGAGCAGGCATATTGCAGGACTATTTACAATGA

Structures

Toxin

• PDB: 5K98
• PDB: 3TPV
• PDB: 3DNV
• PDB: 3TPD
• PDB: 3HZI
• PDB: 3FBR
• PDB: 4YG7
• PDB: 3TPE
• PDB: 2WIU
• PDB: 3DNT
• PDB: 3TPB
• PDB: 3TPT
• PDB: 3DNU