Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 977660..978243 Replicon chromosome
Accession NZ_CP046025
Organism Escherichia coli strain C439

Toxin (Protein)


Gene name mazF Uniprot ID S1EZP4
Locus tag GJH08_RS04650 Protein ID WP_000254738.1
Coordinates 977908..978243 (+) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S1P3W5
Locus tag GJH08_RS04645 Protein ID WP_000581937.1
Coordinates 977660..977908 (+) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GJH08_RS04635 973999..975300 + 1302 WP_000046812.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -
GJH08_RS04640 975348..977582 + 2235 WP_000226815.1 GTP pyrophosphokinase -
GJH08_RS04645 977660..977908 + 249 WP_000581937.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
GJH08_RS04650 977908..978243 + 336 WP_000254738.1 endoribonuclease MazF Toxin
GJH08_RS04655 978314..979105 + 792 WP_001071648.1 nucleoside triphosphate pyrophosphohydrolase -
GJH08_RS04660 979333..980970 + 1638 WP_000210878.1 CTP synthase (glutamine hydrolyzing) -
GJH08_RS04665 981058..982356 + 1299 WP_000036723.1 phosphopyruvate hydratase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-109)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12098.04 Da        Isoelectric Point: 8.2618

>T143401 WP_000254738.1 NZ_CP046025:977908-978243 [Escherichia coli]
MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

>T143401 NZ_CP061081:c596483-596196 [Marinomonas arctica]
ATGAAAGTAAGTTGGTCACCTCTCGCTATGGAAAAGCTTGGGCATGCTGCCGATTTTATCGCTCTAGATAATCCTAACGC
AGCAGAAAAATGGGTAAACGACCTATTCGATAAAACAGACCTACTCAGCCGCCAGCCTGAAATGGGACGAATCGTACCAG
AACTACAAAAAGCAAACCTCCGTGAACTCATTTTTGGTAACTATCGAGTCATCTATACTATAAGTACCGAGATTCGAATA
TTAACCGTACGAAACTGCCGACAGATGCTCACAGAAAGTGATTTATAA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9355.74 Da        Isoelectric Point: 4.4665

>AT143401 WP_000581937.1 NZ_CP046025:977660-977908 [Escherichia coli]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp

>AT143401 NZ_CP061081:c596761-596480 [Marinomonas arctica]
ATGAGCCGTATAAATTTAGATCAAGACATACAACCGTTATCAGAATTTCGTTCTGGTGTGACGTCATTCATTAAACAAAT
TAACGAAACTCGCCGCCCTTTGCTCATCACGCAACGTGGCAAAGGTGTCGCTGTCATGCTCGATGTGGCTGAATACGAAG
CCATGCAAGAAAAAATAGAATTACTCGAAGAAATACGCACCGCTGAATCACAGTTTGCCGAAGGATTAGGCATTTCGAAT
CAGGAAGCAAGAAAGCAAATCTTAGGACGCTTGAAAAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CK9
PDB 5CKH
PDB 5CO7
PDB 3NFC
PDB 5CR2
PDB 5CKB
PDB 5CQY
PDB 5CKD
PDB 5CKE
PDB 5CKF

Antitoxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CQY
PDB 1MVF
PDB 2MRN
PDB 2MRU
AlphaFold DB A0A7U9LMB4

References