Detailed information of TA system TA143393

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ImmA-IrrE/HTH_19(antitoxin)
• Location: 2696171..2697204
• Replicon: chromosome
• Accession: NZ_CP046022
• Organism: Enterococcus faecalis strain BFF1B1

Toxin (Protein)

• Gene name: IrrE
• Uniprot ID: -
• Locus tag: GIO26_RS12810
• Protein ID: WP_174112997.1
• Coordinates: 2696171..2696824 (-)
• Length: 218 a.a.

Antitoxin (Protein)

• Gene name: ImmA
• Uniprot ID: -
• Locus tag: GIO26_RS12815
• Protein ID: WP_010824178.1
• Coordinates: 2696821..2697204 (-)
• Length: 128 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GIO26_RS12790	2691334..2692107	+	774	WP_174113029.1	DUF1295 domain-containing protein	-
GIO26_RS12795	2692423..2694030	-	1608	WP_142477124.1	MucBP domain-containing protein	-
GIO26_RS12800	2694428..2695759	+	1332	WP_174115031.1	FAD-dependent oxidoreductase	-
GIO26_RS12805	2695797..2696132	-	336	WP_002402309.1	helix-turn-helix transcriptional regulator	-
GIO26_RS12810	2696171..2696824	-	654	WP_174112997.1	ImmA/IrrE family metallo-endopeptidase	Toxin
GIO26_RS12815	2696821..2697204	-	384	WP_010824178.1	helix-turn-helix transcriptional regulator	Antitoxin
GIO26_RS12820	2697942..2698193	+	252	WP_033596700.1	hypothetical protein	-
GIO26_RS12825	2698279..2698605	+	327	WP_048949394.1	hypothetical protein	-
GIO26_RS12830	2698605..2699021	+	417	WP_002365225.1	DUF961 family protein	-
GIO26_RS12835	2699191..2699379	-	189	WP_002365223.1	hypothetical protein	-
GIO26_RS12840	2699611..2699835	+	225	WP_002365222.1	hypothetical protein	-
GIO26_RS12845	2699862..2700014	+	153	WP_002399525.1	hypothetical protein	-
GIO26_RS12850	2700025..2700264	+	240	WP_207578930.1	hypothetical protein	-
GIO26_RS12855	2700304..2700627	+	324	WP_142477232.1	hypothetical protein	-
GIO26_RS12860	2700687..2701019	+	333	WP_002399122.1	nucleotidyltransferase domain-containing protein	-
GIO26_RS12865	2701003..2701371	+	369	WP_207578931.1	DUF86 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	2687106..2710625	23519

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• IrrE N-terminal-like domain (75-181)

Antitoxin

• Cro/C1-type helix-turn-helix domain (5-63)

Sequences

Toxin

Protein

• Length: 218 a.a.
• Molecular weight: 26077.07 Da
• Isoelectric Point: 6.7557

>T143393 WP_174112997.1 NZ_CP046022:c2696824-2696171 [Enterococcus faecalis]
MIGLLLVNFISKELYFEAFNFSNELIKEVSYYSSKNIEQVKCFDIEKYVKEVEDVEFVEYTFQKQLKRRMLGSISKVDNE
VIITTNKELMLERKNFTKMHEIMHYYIDIPKINNATHTFSDILLKNGYLMEDFPKEYRANVGASMLMANDQALFYALKKF
YSFEEIAQYFFMSKSALRNRLIEHLMYVNNCTFAHANTLFNNYYFHKETDIYRFIFN

Nucleotide

• Length: 654 bp

>T143393 NZ_CP061080:c2280679-2280425 [Mesirhizobium sp. NIBR3]
ATGCGGATCCTGTGGGACGAACCGAAGCGACTGGCGAACATCGACAAGCATGGCCTCGACTTCTCCGCCCTGACCGAGGA
CTTCTTCGCGTCGGCGGTGATCCGGGTCGCGCGGGGCGAACGCTATCTCGCGGTCGGGGAACTGAGCAGTTTCTTCGTCG
CGGTCGTGTTCCGGCCGCTTGGAGCGGAAGCCCTTTCCGTGATCAGCATGCGGCCGGCCAACAGGAAGGAGACACCGCCA
TATGACGCCTCGTAA

Antitoxin

Protein

• Length: 128 a.a.
• Molecular weight: 15032.08 Da
• Isoelectric Point: 5.3750

>AT143393 WP_010824178.1 NZ_CP046022:c2697204-2696821 [Enterococcus faecalis]
VQPLAKRLQLLREEKEWTKTYVAKQLGLNNLGTYANWEYGTREPDSEMLSKIASLYDVSTDFLLGRTEERIHLNDKEKIG
KNIISHFRLNTSDMDIEDIEELEEELIDFQDFLIKKAKEKKKRNKKD

Nucleotide

• Length: 384 bp

>AT143393 NZ_CP061080:c2280438-2280133 [Mesirhizobium sp. NIBR3]
ATGACGCCTCGTAAGCTTCAGGCGGAGTTCAAGCCCGGACGCGGCTACAGCCGCGAGGATTGGGACGCGGTCTCCGACAA
CCCCGAATGGACCGAGGAGGATTTCGCCCGGGCGCGGCCGTTCGCGGATGTGTTCCCCGAACTCGCCGCCAGCATCCGGC
GCGGGCGGCCGCCGGTGGAGAACCCCAAGAAGCAAGTGACGCTGCGGCTCGATGCGGACGTCGTCGAGCGCTTCCGGGCC
GGCGGGCCGGGGTGGCAGAGCCGCATGAACGAGGCACTGCGCAAGGCGGCCGGCCTCGCGGATTGA