Detailed information of TA system TA143369

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 31320..31978
• Replicon: plasmid unnamed1
• Accession: NZ_CP046020
• Organism: Xanthomonas citri pv. malvacearum strain HD-1

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: Q8PRH3
• Locus tag: GH913_RS22780
• Protein ID: WP_011053002.1
• Coordinates: 31320..31721 (-)
• Length: 134 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: Q3C002
• Locus tag: GH913_RS22785
• Protein ID: WP_005917852.1
• Coordinates: 31718..31978 (-)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GH913_RS22765	28332..29558	-	1227	WP_089504549.1	replication protein	-
GH913_RS22770	29838..31025	-	1188	WP_078566343.1	zeta toxin family protein	-
GH913_RS22775	31032..31295	-	264	WP_005917846.1	hypothetical protein	-
GH913_RS22780	31320..31721	-	402	WP_011053002.1	type II toxin-antitoxin system VapC family toxin	Toxin
GH913_RS22785	31718..31978	-	261	WP_005917852.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
GH913_RS22790	32328..35921	+	3594	WP_095702115.1	TAL effector repeat-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..43707	43707

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-122)

Antitoxin

• SpoVT-AbrB domain (8-52)

Sequences

Toxin

Protein

• Length: 134 a.a.
• Molecular weight: 14627.05 Da
• Isoelectric Point: 7.2785

>T143369 WP_011053002.1 NZ_CP046020:c31721-31320 [Xanthomonas citri pv. malvacearum]
MKRYMLDTNTVSHLVKSHPAVSRRVIEVPMTALCMSAITGGELMFGLAKVPDAKRLQQAVMELLRRVDVLPWDGAVMERY
GSVRADLEKQGKALGSLDMLIAAHALETDSVLVTNDAAFSRVVGLTVEDWTRS

Nucleotide

• Length: 402 bp

>T143369 NZ_CP061071:719547-719738 [Streptococcus mutans B04Sm5]
TTGCCATTAACAGGGAAAGAATTAGCTAGATTAGCGATAAACAATGGATGGGAAGAAGTTCGGGTGAGAGGAAGTCATCA
TCATTTCAAGAAAGATGGAGTACCTTATATTGTGACGATTCCCATTCATGGAAATAAAGTGCTTAAAATTGGTCTTGAAA
AGAAACTCTTAAGGGATTTAAATTTATTATGA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9770.95 Da
• Isoelectric Point: 5.0941

>AT143369 WP_005917852.1 NZ_CP046020:c31978-31718 [Xanthomonas citri pv. malvacearum]
VSQVAKLFINGRSQAVRLPAAYRFTEKEVFIRQDPETGDVILSRKPATWEDFFIALKSADVPANFLDAAERNQGTQDRDP
FAGWRE

Nucleotide

• Length: 261 bp

>AT143369 NZ_CP061071:719756-720133 [Streptococcus mutans B04Sm5]
ATGTTAAAATCATATCCTGCAATTTTTCATAAGGAAGAGGAAGGGTATTGGGTTGAATTTCCTGAATTTGGCGGTGGTAC
GCAAGGGGAAGATTTGGAAGAAGCCATGAAGAACGCTCGTCAGATGTTAGAAAGTGTATTGGCCTCCTATCTAGATGAGG
GAATGAAACTGCCTAATCCAAGTGAGATAAGGAAACTATCTGTTGAAGATGGCTTTGCAACTATGATTCAAGCAGATCCT
AATCCTTATCTCAAAAATAACAAAGCTATTCGAAAGAATGTTACCGTACCTGAGTGGCTAGTACAATTAGCAGATCGTGA
TCAAGTGAATTATTCTGAAGTATTAACAAAGGCTTTGGAAAAGAAACTACAATTATAA

Structures

Toxin

• AlphaFold DB: Q8PRH3

Antitoxin

• AlphaFold DB: Q3C002