Detailed information of TA system TA143366

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK-MazE
• Location: 921889..922430
• Replicon: chromosome
• Accession: NZ_CP046019
• Organism: Xanthomonas citri pv. malvacearum strain HD-1

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: GH913_RS03940
• Protein ID: WP_003491151.1
• Coordinates: 921889..922215 (-)
• Length: 109 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: H8FMA7
• Locus tag: GH913_RS03945
• Protein ID: WP_003491150.1
• Coordinates: 922212..922430 (-)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GH913_RS23045	917080..917253	+	174	WP_164722239.1	hypothetical protein	-
GH913_RS03930	917257..918831	+	1575	WP_095702034.1	type IV secretion system DNA-binding domain-containing protein	-
GH913_RS03935	918845..921820	+	2976	WP_089504402.1	conjugative relaxase	-
GH913_RS03940	921889..922215	-	327	WP_003491151.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
GH913_RS03945	922212..922430	-	219	WP_003491150.1	antitoxin MazE family protein	Antitoxin
GH913_RS03950	922573..922959	-	387	WP_003491149.1	hypothetical protein	-
GH913_RS03955	923036..923455	-	420	WP_078566256.1	hypothetical protein	-
GH913_RS03960	923581..924450	-	870	WP_078566255.1	transglycosylase SLT domain-containing protein	-
GH913_RS03965	924461..925483	-	1023	WP_154138456.1	P-type DNA transfer ATPase VirB11	-
GH913_RS03970	925494..926714	-	1221	WP_154138457.1	TrbI/VirB10 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	898546..934857	36311

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (2-105)

Antitoxin

• Antitoxin MazE-like (5-70)

Sequences

Toxin

Protein

• Length: 109 a.a.
• Molecular weight: 11690.73 Da
• Isoelectric Point: 9.2550

>T143366 WP_003491151.1 NZ_CP046019:c922215-921889 [Xanthomonas citri pv. malvacearum]
MMRGDFVTIAMQGDFGKPRPALVIQADQFDAHTTVTVLPVTSTLVAAPLLRITIHPSTDNGLQKPSQVMVDKAMTVKRDK
VGRAFGRVDADALVEIERCLAVFLGIAK

Nucleotide

• Length: 327 bp

>T143366 NZ_CP061062:5450678-5451088 [Klebsiella pneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTTCACGGCGTGGTGGCGGC
CCTGGTGCTGTTGGTTCCCTGGCCGCTGAGCTATACCCCGATCTGGCTGCTGCTGCTGTCGCTGGTGGTCTTCGACTGCG
TGCGCAGCCAGCGGCGGATCCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCC
GAATGGGAGATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGGCGCGG
ACAGCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGGAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGG
CGCAGGAGTAA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8418.54 Da
• Isoelectric Point: 5.6599

>AT143366 WP_003491150.1 NZ_CP046019:c922430-922212 [Xanthomonas citri pv. malvacearum]
MAHVNSRVQKHRDALRMAGLRPVQIWVPDTRRPDFAEECRRQCRLAAQADMADTDMQRFMDEALADMDGWTE

Nucleotide

• Length: 219 bp

>AT143366 NZ_CP061062:5450431-5450697 [Klebsiella pneumoniae]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Antitoxin

• AlphaFold DB: H8FMA7