Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3432031..3432649 Replicon chromosome
Accession NZ_CP046009
Organism Escherichia coli strain 1919D62

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag GJD96_RS18140 Protein ID WP_001291435.1
Coordinates 3432431..3432649 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag GJD96_RS18135 Protein ID WP_000344800.1
Coordinates 3432031..3432405 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GJD96_RS18125 3427120..3428313 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
GJD96_RS18130 3428336..3431485 + 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
GJD96_RS18135 3432031..3432405 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
GJD96_RS18140 3432431..3432649 + 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
GJD96_RS18145 3432821..3433372 + 552 WP_154167091.1 maltose O-acetyltransferase -
GJD96_RS18150 3433488..3433958 + 471 WP_000136192.1 YlaC family protein -
GJD96_RS18155 3434122..3435672 + 1551 WP_001310610.1 cyclic-guanylate-specific phosphodiesterase PdeB -
GJD96_RS18160 3435714..3436067 - 354 WP_000878140.1 DUF1428 domain-containing protein -
GJD96_RS18170 3436446..3436757 + 312 WP_000409911.1 MGMT family protein -
GJD96_RS18175 3436788..3437360 - 573 WP_000779826.1 lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T143359 WP_001291435.1 NZ_CP046009:3432431-3432649 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T143359 NZ_CP061062:3301842-3302060 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACCTCTGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT143359 WP_000344800.1 NZ_CP046009:3432031-3432405 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT143359 NZ_CP061062:3301441-3301815 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References