Detailed information of TA system TA143347

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 1356113..1356738
• Replicon: chromosome
• Accession: NZ_CP046009
• Organism: Escherichia coli strain 1919D62

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: GJD96_RS08230
• Protein ID: WP_000911330.1
• Coordinates: 1356340..1356738 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: GJD96_RS08225
• Protein ID: WP_000450524.1
• Coordinates: 1356113..1356340 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GJD96_RS08200	1351916..1352386	-	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-
GJD96_RS08205	1352386..1352958	-	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
GJD96_RS08210	1353104..1353982	+	879	WP_001311023.1	4-hydroxy-tetrahydrodipicolinate synthase	-
GJD96_RS08215	1353999..1355033	+	1035	WP_001297320.1	outer membrane protein assembly factor BamC	-
GJD96_RS08220	1355246..1355959	+	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
GJD96_RS08225	1356113..1356340	+	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
GJD96_RS08230	1356340..1356738	+	399	WP_000911330.1	tRNA(fMet)-specific endonuclease VapC	Toxin
GJD96_RS08235	1356885..1357748	+	864	WP_001267498.1	neutral zinc metallopeptidase	-
GJD96_RS08240	1357763..1359778	+	2016	WP_154166972.1	tRNA cytosine(34) acetyltransferase TmcA	-
GJD96_RS08245	1359852..1360550	+	699	WP_000679812.1	esterase	-
GJD96_RS08250	1360660..1360860	-	201	WP_000383836.1	YpfN family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T143347 WP_000911330.1 NZ_CP046009:1356340-1356738 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T143347 NZ_CP061053:1472555-1473037 [Klebsiella pneumoniae]
GTGGGACGTGTAACCGCGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTTGA
TGAATGGCTAAAGCACAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGGACGA
AGCAAGTAGCTGGTTTTTACTCTCTGGCCACCGGTAGCGTTAACCATACGGAAACTACAGGCAGACTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTTCACGATGCCGTTCTACGCTGTTATCGCGTAGCCGAGAATATTGGCGTCCGTGCGATAATGGTTCACGCGCTTACTG
AAGAAGCTAAAGGCTTCTATACTCACCATGGATTTACGGCATCGCAAACTCATGAGCGGACTCTGTTCCTTAAACTTCCA
TGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT143347 WP_000450524.1 NZ_CP046009:1356113-1356340 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT143347 NZ_CP061053:1472301-1472567 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCCGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCTTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTGTAA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33