Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 898901..899555 Replicon chromosome
Accession NZ_CP046009
Organism Escherichia coli strain 1919D62

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag GJD96_RS06005 Protein ID WP_000244777.1
Coordinates 899148..899555 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag GJD96_RS06000 Protein ID WP_000354046.1
Coordinates 898901..899167 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GJD96_RS05975 894070..894813 + 744 WP_000951948.1 SDR family oxidoreductase -
GJD96_RS05980 894870..896303 - 1434 WP_053900613.1 6-phospho-beta-glucosidase BglA -
GJD96_RS05985 896348..896659 + 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
GJD96_RS05990 896823..897482 + 660 WP_000250274.1 hemolysin III family protein -
GJD96_RS05995 897678..898658 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
GJD96_RS06000 898901..899167 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
GJD96_RS06005 899148..899555 + 408 WP_000244777.1 toxin CptA Toxin
GJD96_RS06010 899595..900116 - 522 WP_001055874.1 flavodoxin FldB -
GJD96_RS06015 900228..901124 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
GJD96_RS06020 901149..901859 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
GJD96_RS06025 901865..903598 + 1734 WP_000813212.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T143345 WP_000244777.1 NZ_CP046009:899148-899555 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T143345 NZ_CP061053:836222-836632 [Klebsiella pneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTTCACGGCGTGGTGGCGGC
CCTGGTGCTGTTGGTTCCCTGGCCGCTGAGCTATACCCCGATCTGGCTGCTGCTGCTGTCGCTGGTGGTCTTCGACTGCG
TGCGCAGCCAGCGGCGGATCCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCC
GAATGGGAGATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGGCGCGG
ACAGCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGGAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGG
CGCAGGAGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT143345 WP_000354046.1 NZ_CP046009:898901-899167 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT143345 NZ_CP061053:835975-836241 [Klebsiella pneumoniae]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References