Detailed information of TA system TA143344

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataT-KacA/DUF1778(antitoxin)
• Location: 17593..18391
• Replicon: plasmid p1919D62-2
• Accession: NZ_CP046008
• Organism: Escherichia coli strain 1919D62

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: -
• Locus tag: GJD96_RS01120
• Protein ID: WP_033870689.1
• Coordinates: 17593..18114 (-)
• Length: 174 a.a.

Antitoxin (Protein)

• Gene name: KacA
• Uniprot ID: A0A141BRA2
• Locus tag: GJD96_RS01125
• Protein ID: WP_001711191.1
• Coordinates: 18122..18391 (-)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GJD96_RS01095	14103..15218	+	1116	WP_124884819.1	tail fiber domain-containing protein	-
GJD96_RS01100	15281..16066	+	786	WP_096098950.1	receptor-recognizing protein	-
GJD96_RS01105	16141..16464	+	324	WP_001711185.1	hypothetical protein	-
GJD96_RS01110	16478..17170	+	693	WP_033546097.1	membrane protein	-
GJD96_RS01115	17173..17424	+	252	WP_023135660.1	hypothetical protein	-
GJD96_RS01120	17593..18114	-	522	WP_033870689.1	GNAT family N-acetyltransferase	Toxin
GJD96_RS01125	18122..18391	-	270	WP_001711191.1	DUF1778 domain-containing protein	Antitoxin
GJD96_RS01130	18711..19379	+	669	WP_000161228.1	AAA family ATPase	-
GJD96_RS01135	19385..19738	+	354	WP_160378290.1	hypothetical protein	-
GJD96_RS01140	19791..20558	-	768	WP_033870691.1	hypothetical protein	-
GJD96_RS01145	20841..21566	+	726	WP_023135656.1	hypothetical protein	-
GJD96_RS01150	21627..22967	+	1341	WP_063122790.1	AAA family ATPase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..114129	114129

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (48-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (8-86)

Sequences

Toxin

Protein

• Length: 174 a.a.
• Molecular weight: 19395.22 Da
• Isoelectric Point: 8.2535

>T143344 WP_033870689.1 NZ_CP046008:c18114-17593 [Escherichia coli]
VSNTTIEIFSGEKDYDLNGFDCGEESLNAFLTNHLKRQHEGKILRAYVLCTKEEIPKVLGYYTLSGSCFEKENLPSKSQQ
KKVPYRHVPSITLGRLALDKSLQGQGLGSMLVTHAMRVVYNASLAVGIHGLFVEALNDKARAFYKSLGFIQLVGNNDRSL
FYPTKSIEKLFEE

Nucleotide

• Length: 522 bp

>T143344 NZ_CP061053:358784-359131 [Klebsiella pneumoniae]
ATGTGGGATGTGGAAACGACGGATACGTTTGATGCCTGGTTCGAATTACAAAGTAGAGCTTTAAAAGAGGATATGTTGGC
CACGATGCTCATCTTGTCTGAGTTTGCTCCGCAGCTTGGGCGCCCGTATGTTGATACGGTGAAAGATTCAACGTTTCAGA
ATATGAAAGAACTGCGGGTTCAGCATCATGGGCTCCCCATCCGCGCCTTTTTTGCTTTTGATCCTCTGCGGAAAGCTATC
GTTTTATGTGCGGGCAATAAGGATGGCATAAATGAGAAACGTTTTTACAAAGAGATGATTACTCTGGCTGACAGAGAATT
CAGCCAACACCTGACTAAGGAGCGATAA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10054.53 Da
• Isoelectric Point: 8.7353

>AT143344 WP_001711191.1 NZ_CP046008:c18391-18122 [Escherichia coli]
MSALKKQRIDLRLTDDDKSIIEEAAAMSNQSITQFMVSSASERAVKVIEQHRRLVLSEESWNLVMDAISNPPAPNDKLKR
AANRLKSME

Nucleotide

• Length: 270 bp

>AT143344 NZ_CP061053:359131-359430 [Klebsiella pneumoniae]
ATGGCCACGCTAAAAGAACTCATGGCGAAACAGAGTCCTGAAAGCCAGCAGCGTATTGCAGCGAAAGCGGCAGAGATACG
CCAGTCTGTCGCTTTAAACTTGCTTCGAGAAGAGTTGCAAATGTCGCAAACAGAGATGGCTGCGGCTATGGGGGTAAAGC
AGCCAACGATCGCTAAAATGGAACAGACTGATAACGACCCTCGGCTTTCGACCCTAAAAAGCTACATTGCGGCATTGGGG
GGAGAACTCAGTATAAATGTCACATTGCCGACTGGAAAAAAAGTCTCGTTTAATTTGTGA

Structures

Antitoxin

• AlphaFold DB: A0A141BRA2