Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4034127..4034703 Replicon chromosome
Accession NZ_CP046006
Organism Escherichia coli strain 1919D3

Toxin (Protein)


Gene name shpA Uniprot ID A0A0E2LC92
Locus tag GJD95_RS20780 Protein ID WP_001299718.1
Coordinates 4034416..4034703 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A7U9DAW4
Locus tag GJD95_RS20775 Protein ID WP_000063150.1
Coordinates 4034127..4034429 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GJD95_RS20755 4029818..4030330 + 513 WP_001175455.1 4-hydroxyphenylacetate 3-monooxygenase reductase subunit -
GJD95_RS20760 4030723..4032873 + 2151 WP_040072211.1 pyruvate/proton symporter BtsT -
GJD95_RS20765 4032923..4033126 + 204 WP_000467859.1 YbdD/YjiX family protein -
GJD95_RS20770 4033137..4034093 + 957 WP_154166467.1 GTPase -
GJD95_RS20775 4034127..4034429 - 303 WP_000063150.1 BrnA antitoxin family protein Antitoxin
GJD95_RS20780 4034416..4034703 - 288 WP_001299718.1 BrnT family toxin Toxin
GJD95_RS20785 4034900..4036066 + 1167 WP_096310305.1 restriction endonuclease -
GJD95_RS20790 4036130..4037422 + 1293 Protein_3739 SAM-dependent DNA methyltransferase -
GJD95_RS20795 4037488..4038650 + 1163 WP_154166468.1 IS3-like element IS3 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11142.60 Da        Isoelectric Point: 7.4697

>T143341 WP_001299718.1 NZ_CP046006:c4034703-4034416 [Escherichia coli]
MPMEFEWDANKAKSNRVKHGIRFEDAVLVFDDPQHLSQQDRIENGEYRWQTIGVVHGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERSRYEHG

Download         Length: 288 bp

>T143341 NZ_CP061048:24579-24977 [Salmonella enterica subsp. enterica serovar Typhimurium]
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11532.03 Da        Isoelectric Point: 9.4646

>AT143341 WP_000063150.1 NZ_CP046006:c4034429-4034127 [Escherichia coli]
MSMVKHKRGTPSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT143341 NZ_CP061048:24352-24579 [Salmonella enterica subsp. enterica serovar Typhimurium]
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E2LC92

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9DAW4

References