Detailed information of TA system TA143335

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 2295725..2297313
• Replicon: chromosome
• Accession: NZ_CP046006
• Organism: Escherichia coli strain 1919D3

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: F4NFZ0
• Locus tag: GJD95_RS12590
• Protein ID: WP_001125463.1
• Coordinates: 2295991..2297313 (+)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: GJD95_RS12585
• Protein ID: WP_001296726.1
• Coordinates: 2295725..2295991 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GJD95_RS12585	2295725..2295991	+	267	WP_001296726.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
GJD95_RS12590	2295991..2297313	+	1323	WP_001125463.1	type II toxin-antitoxin system serine/threonine protein kinase toxin HipA	Toxin
GJD95_RS12595	2297600..2298547	-	948	WP_154166423.1	hypothetical protein	-
GJD95_RS12600	2299362..2300453	-	1092	WP_061359330.1	hypothetical protein	-
GJD95_RS12605	2300848..2301056	+	209	Protein_2126	helix-turn-helix transcriptional regulator	-
GJD95_RS12610	2301231..2301928	+	698	WP_154166424.1	IS1 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (7-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-69)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49422.05 Da
• Isoelectric Point: 9.1203

>T143335 WP_001125463.1 NZ_CP046006:2295991-2297313 [Escherichia coli]
MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLASRYARPLSLSLPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYH
AKSRQPFDLLSEIGRDSVGAVTLLPENETITRPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVPDAEIIKAGRVRALAVERFDRRW
NTERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIARIMAFLMGSSEALRDRYDFMKFQVFQWLIGATDGHAKNFSVFIQ
AGGSYRLTPFYDIISAFPVLGGTGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLRFPEVQMHEILSDFARMIP
AALDNVKTSLPTDFPENVVTAVETNVLRLHGRLSREYGSK

Nucleotide

• Length: 1323 bp

>T143335 NZ_CP061041:c510236-509874 [Staphylococcus warneri]
ATGATTAGAAGAGGAGATGTTTATCTAGCAGATTTATCACCTGTTCAAGGGTCAGAACAAGGGGGAGTAAGACCCGTCGT
CATTATCCAAAATGACACAGGTAATAAATATAGTCCTACTGTTATTGTTGCCGCAATCACTGGAAGGATTAATAAAGCTA
AGATACCTACACATGTTGAAATTGAAAAGAAAAAGTACAAACTTGATAAGGACTCAGTTATTTTATTAGAACAAATTCGT
ACTTTAGATAAAAATCGTTTAAAAGAAAAACTCACATATTTGTCTGATAACAAAATGAAAGAAGTAGATTACGCCCTAGA
CATCAGTCTAGGTTTACATAATTTCCATCATTCTAAGACATAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10047.39 Da
• Isoelectric Point: 4.9632

>AT143335 WP_001296726.1 NZ_CP046006:2295725-2295991 [Escherichia coli]
MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISNFENNPDNTTLTTFFKILQSLELSMTLCDTKNASPES
TEQQDLEW

Nucleotide

• Length: 267 bp

>AT143335 NZ_CP061041:c510403-510233 [Staphylococcus warneri]
ATGTTATCTTTTAATCAAAATAGAAGTTACAGTTTAGAGCAATCTTTAAAAGAAGGTTATGCACAAATGGCTGATTTAAA
TCTCTCCCTAGCAACCGAAGCTTTTCCTATTGAATGTGAAGCGTGCGATTGCAATGAAACATATTTAACTTCTAACGCTA
AGAATGAATGA

Structures

Toxin

• AlphaFold DB: A0A7U9IYX0