Detailed information of TA system TA143320

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 49624..50459
• Replicon: chromosome
• Accession: NZ_CP046006
• Organism: Escherichia coli strain 1919D3

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: GJD95_RS01965
• Protein ID: WP_154166362.1
• Coordinates: 49624..50001 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A369FMF5
• Locus tag: GJD95_RS01970
• Protein ID: WP_024188924.1
• Coordinates: 50091..50459 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GJD95_RS01935	44989..45912	-	924	WP_000535960.1	carboxylate/amino acid/amine transporter	-
GJD95_RS01940	46023..47207	-	1185	WP_097433495.1	sugar efflux transporter	-
GJD95_RS01950	48000..48845	-	846	WP_000065752.1	DUF4942 domain-containing protein	-
GJD95_RS01955	48930..49127	-	198	WP_000839243.1	DUF957 domain-containing protein	-
GJD95_RS01960	49139..49627	-	489	WP_154166361.1	hypothetical protein	-
GJD95_RS01965	49624..50001	-	378	WP_154166362.1	TA system toxin CbtA family protein	Toxin
GJD95_RS01970	50091..50459	-	369	WP_024188924.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
GJD95_RS01975	50533..50754	-	222	WP_073544043.1	DUF987 domain-containing protein	-
GJD95_RS01980	50823..51299	-	477	WP_143359950.1	RadC family protein	-
GJD95_RS01985	51315..51800	-	486	WP_000214415.1	antirestriction protein	-
GJD95_RS01990	51892..52710	-	819	WP_072643846.1	DUF945 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (19-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14105.10 Da
• Isoelectric Point: 7.3225

>T143320 WP_154166362.1 NZ_CP046006:c50001-49624 [Escherichia coli]
MNTLPDTHVREASRCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQLGF
SAGAPSQLINSIDILRARRATGLMTRNNYRMVNNITQGMHPEAKR

Nucleotide

• Length: 378 bp

>T143320 NZ_CP061034:c144092-143811 [Pseudomonas aeruginosa]
ATGAGCCTGAAGTGGACCCGCAAGGCGGCCGCCGACCTGGACGCCATCTACGACCATTACGTCGTGCTGATCGGCCCGGA
AAAAGCTCTGAAAGCCGTTCAGGACATCGTCGAGCAGGTGAAACCGCTGCAGCAGGTAGCCAACCAGGGGGCAGGGCGGC
CCAGCGAGGTGCCAGGCGTACGCACCCTGACCCTGGAGCGCTGGCCGTTCAGCGCCCCGTTTCGGGTTAAAGGCAAGGAA
ATCCAGATTTTGCGCATCGACAGGGTCGAAATTACCCCCTGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13633.45 Da
• Isoelectric Point: 6.7386

>AT143320 WP_024188924.1 NZ_CP046006:c50459-50091 [Escherichia coli]
VSDTLPGTTHPDDNHDRLWWGLPCTVTPCFGARLVQKGNRLHYLADRAGIRGRFSDADTYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKT

Nucleotide

• Length: 369 bp

>AT143320 NZ_CP061034:c144316-144089 [Pseudomonas aeruginosa]
ATGAGCACTGTAGTCTCGTTCCGCGCCGATGACGCCCTGGTCGCGGCCCTCGACGAACTGGCCCGCGCCACCCACCGCGA
CCGCCCCTACCACCTGCGGCAGGCGCTCGCGCAGTACCTGGAAAGGCAGCAGTGGCAGGTCGCTGCCATCGATGAAGGCT
TGGCCGATGCCAATGCCGGTCGCCTGCTGGAACACACCGAGATCGAGAAGCGCTGGGGGCTGCAATGA

Structures

Antitoxin

• AlphaFold DB: A0A369FMF5