Detailed information of TA system TA143319

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-Phd
• Location: 38585..39186
• Replicon: plasmid p1919D3-2
• Accession: NZ_CP046005
• Organism: Escherichia coli strain 1919D3

Toxin (Protein)

• Gene name: doc
• Uniprot ID: -
• Locus tag: GJD95_RS01445
• Protein ID: WP_024233187.1
• Coordinates: 38806..39186 (+)
• Length: 127 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: U9YQH9
• Locus tag: GJD95_RS01440
• Protein ID: WP_001190712.1
• Coordinates: 38585..38806 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GJD95_RS01430	35575..36849	-	1275	WP_154166353.1	restriction endonuclease subunit S	-
GJD95_RS01435	36846..38402	-	1557	WP_072716651.1	type I restriction-modification system subunit M	-
GJD95_RS01440	38585..38806	+	222	WP_001190712.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
GJD95_RS01445	38806..39186	+	381	WP_024233187.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
GJD95_RS01450	39191..39370	+	180	WP_009446826.1	hypothetical protein	-
GJD95_RS01455	39398..40441	+	1044	WP_024233186.1	DUF968 domain-containing protein	-
GJD95_RS01460	40530..40982	+	453	WP_001326849.1	late promoter-activating protein	-
GJD95_RS01465	41068..42261	+	1194	WP_024233185.1	hypothetical protein	-
GJD95_RS01470	42261..43745	+	1485	WP_032163774.1	hypothetical protein	-
GJD95_RS01475	43967..44086	+	120	WP_071527722.1	ash family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..95543	95543

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (2-123)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-51)

Sequences

Toxin

Protein

• Length: 127 a.a.
• Molecular weight: 13574.27 Da
• Isoelectric Point: 5.1408

>T143319 WP_024233187.1 NZ_CP046005:38806-39186 [Escherichia coli]
MRHISPEELIALHDANISRYGGLPGMSDPGRAEAIIGRVQARVAYEDITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Nucleotide

• Length: 381 bp

>T143319 NZ_CP061032:c1487898-1487647 [Corynebacterium sp. zg-917]
TTGTCTATCAGCGCATCTGAAGCGCGCCGGACGTTGTACCCGCTTATTGAACGAGTGAATAACGACCGCGATGCAGTCGA
GATCGTTTCGCGAAACGGTAACGCAGTGTTGATGGCCGCAGAAGATTACGAGGCCTGGGTGGAGACCGCATACCTCTTTA
AGTCGCGAGAAAATGCACGCCGGCTGACACGATCCTACGATGACGCGCTTAACGGCGCAGTGTCCGAACACGACGTTGAT
TTGGATGCATAG

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8133.10 Da
• Isoelectric Point: 4.7708

>AT143319 WP_001190712.1 NZ_CP046005:38585-38806 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Nucleotide

• Length: 222 bp

>AT143319 NZ_CP061032:c1487647-1487393 [Corynebacterium sp. zg-917]
GTGCGTCTGGTTTGGACAGACGATGCCTGGAGCGATTACCTCCATTGGCAGAGTAATGACCGGAAAACACTCAAACGTGT
GAACCAACTGCTGAAAGATATGTTGCGAGATCCGTTTGAAGGAATAGGCAAGCCCGAGCCACTCAAATACGGTGCTGAAG
GTGCGTGGTCAAGGAGGATCACGCAAGAGCACCGCCTGGTGTACATGGTTTCCGGGGGAGACCTGGTGATCCTGCAGGCT
CGATACCACTACTAA

Structures

Antitoxin

• AlphaFold DB: A0A829CJB6