Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3462599..3463217 Replicon chromosome
Accession NZ_CP046003
Organism Escherichia coli strain 1916D6

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag GJD94_RS17415 Protein ID WP_001291435.1
Coordinates 3462999..3463217 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag GJD94_RS17410 Protein ID WP_000344800.1
Coordinates 3462599..3462973 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GJD94_RS17400 3457688..3458881 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
GJD94_RS17405 3458904..3462053 + 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
GJD94_RS17410 3462599..3462973 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
GJD94_RS17415 3462999..3463217 + 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
GJD94_RS17420 3463389..3463940 + 552 WP_000102564.1 maltose O-acetyltransferase -
GJD94_RS17425 3464056..3464526 + 471 WP_000136192.1 YlaC family protein -
GJD94_RS17430 3464690..3466240 + 1551 WP_001310610.1 cyclic-guanylate-specific phosphodiesterase PdeB -
GJD94_RS17435 3466282..3466589 - 308 Protein_3265 DUF1428 family protein -
GJD94_RS17445 3466968..3467279 + 312 WP_000409911.1 MGMT family protein -
GJD94_RS17450 3467310..3467882 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T143312 WP_001291435.1 NZ_CP046003:3462999-3463217 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T143312 NZ_CP061030:c318369-318016 [Staphylococcus pseudintermedius]
ATGAAGCGCGGAGACGTTTATTTAGCAGATTTATCACCAGTACAGGGCTCTGAACAGGGGGGAGTAAGGCCCGTTGTCAT
TATTCAAAATGACACTGGTAATAAGTATAGTCCCACTGTGATTGTAGCCGCAATTACTGGTCGGATTAATAAGGCAAAGA
TACCAACGCATGTCGAAATCGAAAAAAATAAATATAAGCTCGATAAAGATTCTGTTATTTTACTAGAACAAATTCGAACA
GTCGATAAGAAACGATTAAAAGAAAAGTTAACGTTTTTGTCTGAGAAGAAGATGAAAGAAGTTAACAATGCGATTGGTAT
TAGTTTAGGACTGCACATGAATCATCATAAATAG

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT143312 WP_000344800.1 NZ_CP046003:3462599-3462973 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT143312 NZ_CP061030:c318540-318370 [Staphylococcus pseudintermedius]
ATGTCAGCATTCAATCAAACAAGATTTAAAAATTTAGAACAGTCGTTAAAAGAAGGTTATGTACAGATGGCAGATTTAAA
TCTCTCCCTAGCCACTGAGGCGTATCCAGTTGAATGTGAAGCTTGCGATTGTAATGAATCACACTTGACAACTGATTACA
AGGAAGACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References