143294

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-DnaT
Location 48562..49084 Replicon plasmid p1916D6-1
Accession NZ_CP046001
Organism Escherichia coli strain 1916D6

Toxin (Protein)


Gene name relE Uniprot ID G3CAN5
Locus tag GJD94_RS00240 Protein ID WP_000220560.1
Coordinates 48562..48843 (-) Length 94 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID G3CAG1
Locus tag GJD94_RS00245 Protein ID WP_000121743.1
Coordinates 48833..49084 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GJD94_RS00215 45841..46038 + 198 WP_000843497.1 DUF2933 domain-containing protein -
GJD94_RS00220 46072..46347 - 276 Protein_43 peptidoglycan DD-metalloendopeptidase family protein -
GJD94_RS00225 46382..47350 + 969 WP_077783444.1 IS5 family transposase -
GJD94_RS00230 47387..47644 - 258 WP_105278499.1 hypothetical protein -
GJD94_RS00235 47790..48487 - 698 Protein_46 IS1 family transposase -
GJD94_RS00240 48562..48843 - 282 WP_000220560.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
GJD94_RS00245 48833..49084 - 252 WP_000121743.1 plasmid stabilization protein Antitoxin
GJD94_RS00250 50318..51154 + 837 WP_001050931.1 replication initiation protein -
GJD94_RS00255 51194..51640 + 447 Protein_50 hypothetical protein -
GJD94_RS00260 51708..52133 + 426 WP_000202942.1 hypothetical protein -
GJD94_RS00265 52130..53107 - 978 WP_024245156.1 hypothetical protein -
GJD94_RS00270 53132..53413 - 282 WP_001185482.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid floR / mef(B) / sul3 / dfrA5 - 1..73506 73506


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 94 a.a.        Molecular weight: 11005.83 Da        Isoelectric Point: 10.5938

>T143294 WP_000220560.1 NZ_CP046001:c48843-48562 [Escherichia coli]
MTYELAFDRRALKEWQKLGHTIREQFKKKLAERLENPRVPAARLHGHADRYKIKLRASGYRLVYQVIDEKVVLLVIAVGR
RESSEVYQIADLR

Download         Length: 282 bp

>T143294 NZ_CP060988:14713-15129 [Escherichia coli]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAGCAGGTGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCT
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACCGCCATTGC
CGGACACGCCATCGCGGCGGGCGCCATCCTGGTGACAAACAATGTGAGAGAGTTTGAGCGGGTGCCGGGTCTGGTTCTGG
AAGACTGGGTTAAGTAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9194.43 Da        Isoelectric Point: 4.1683

>AT143294 WP_000121743.1 NZ_CP046001:c49084-48833 [Escherichia coli]
MSYQILTTTAASITDLKKNPMGTVAEGEGDAVAILNRNEPAFYCVPPKLYAYYRELAEDAELNAVADERMKNPEIVKVNL
DDL

Download         Length: 252 bp

>AT143294 NZ_CP060988:14486-14716 [Escherichia coli]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGTGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGATCCAGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGACGAAGGACGCTTTGAGCCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB G3CAN5

Antitoxin

Source ID Structure
AlphaFold DB A0A3S5I301

References