Detailed information of TA system TA143289

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 3520626..3521463
• Replicon: chromosome
• Accession: NZ_CP046000
• Organism: Escherichia coli strain 1916D18

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: Q3Z4X7
• Locus tag: GJD92_RS17585
• Protein ID: WP_000227784.1
• Coordinates: 3520921..3521463 (+)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: GJD92_RS17580
• Protein ID: WP_001297137.1
• Coordinates: 3520626..3520937 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GJD92_RS17555	3515646..3516593	+	948	WP_001239441.1	cytochrome o ubiquinol oxidase subunit II	-
GJD92_RS17560	3516615..3518606	+	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
GJD92_RS17565	3518596..3519210	+	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
GJD92_RS17570	3519210..3519539	+	330	WP_025649657.1	cytochrome o ubiquinol oxidase subunit IV	-
GJD92_RS17575	3519551..3520441	+	891	WP_000971336.1	heme o synthase	-
GJD92_RS17580	3520626..3520937	+	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
GJD92_RS17585	3520921..3521463	+	543	WP_000227784.1	GNAT family N-acetyltransferase	Toxin
GJD92_RS17590	3521519..3522454	-	936	WP_001297127.1	sel1 repeat family protein	-
GJD92_RS17595	3522862..3524226	+	1365	WP_001000978.1	MFS transporter	-
GJD92_RS17600	3524354..3524845	-	492	WP_001138904.1	nucleotide binding protein YajQ	-
GJD92_RS17605	3525013..3525924	+	912	WP_000705874.1	2-dehydropantoate 2-reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19805.02 Da
• Isoelectric Point: 8.3395

>T143289 WP_000227784.1 NZ_CP046000:3520921-3521463 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T143289 NZ_CP060987:c3910712-3910314 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCCTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT143289 WP_001297137.1 NZ_CP046000:3520626-3520937 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT143289 NZ_CP060987:c3911008-3910715 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• PDB: 7BYY

Antitoxin

• AlphaFold DB: A0A0E0Y6B3