Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 2485672..2486310 Replicon chromosome
Accession NZ_CP046000
Organism Escherichia coli strain 1916D18

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag GJD92_RS12575 Protein ID WP_000813794.1
Coordinates 2486134..2486310 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag GJD92_RS12570 Protein ID WP_001270286.1
Coordinates 2485672..2486088 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GJD92_RS12550 2480824..2481765 - 942 WP_001251315.1 ABC transporter permease -
GJD92_RS12555 2481766..2482779 - 1014 WP_000220411.1 ABC transporter ATP-binding protein -
GJD92_RS12560 2482797..2483942 - 1146 WP_000047456.1 ABC transporter substrate-binding protein -
GJD92_RS12565 2484187..2485593 - 1407 WP_000760629.1 PLP-dependent aminotransferase family protein -
GJD92_RS12570 2485672..2486088 - 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
GJD92_RS12575 2486134..2486310 - 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
GJD92_RS12580 2486532..2486762 + 231 WP_000494244.1 YncJ family protein -
GJD92_RS12585 2486854..2488815 - 1962 WP_001301045.1 23S rRNA 5-hydroxycytidine C2501 synthase -
GJD92_RS12590 2488888..2489424 - 537 WP_000429155.1 DNA-binding transcriptional regulator SutR -
GJD92_RS12595 2489477..2490691 + 1215 WP_001307190.1 BenE family transporter YdcO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T143287 WP_000813794.1 NZ_CP046000:c2486310-2486134 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T143287 NZ_CP060987:2806033-2806140 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCGGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT143287 WP_001270286.1 NZ_CP046000:c2486088-2485672 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT143287 NZ_CP060987:c2805986-2805920 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References