Detailed information of TA system TA143273

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 731296..732116
• Replicon: chromosome
• Accession: NZ_CP046000
• Organism: Escherichia coli strain 1916D18

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: GJD92_RS04230
• Protein ID: WP_154139486.1
• Coordinates: 731739..732116 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: GJD92_RS04225
• Protein ID: WP_174213414.1
• Coordinates: 731296..731649 (+)
• Length: 118 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GJD92_RS04190	726400..727305	+	906	WP_000203518.1	chemotaxis protein	-
GJD92_RS04195	727302..728372	+	1071	WP_000102654.1	patatin-like phospholipase family protein	-
GJD92_RS04200	728763..728936	+	174	WP_000088743.1	DUF905 family protein	-
GJD92_RS04205	729036..729857	+	822	WP_033803192.1	DUF945 domain-containing protein	-
GJD92_RS04210	729949..730434	+	486	WP_021538211.1	antirestriction protein	-
GJD92_RS04215	730450..730926	+	477	WP_154139484.1	RadC family protein	-
GJD92_RS04220	730995..731216	+	222	WP_000692326.1	DUF987 domain-containing protein	-
GJD92_RS04225	731296..731649	+	354	WP_174213414.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
GJD92_RS04230	731739..732116	+	378	WP_154139486.1	type IV toxin-antitoxin system toxin CbtA	Toxin
GJD92_RS04235	732113..732262	+	150	Protein_689	hypothetical protein	-
GJD92_RS04240	732338..732535	+	198	WP_154139595.1	DUF957 domain-containing protein	-
GJD92_RS04245	732620..733465	+	846	WP_001274556.1	DUF4942 domain-containing protein	-
GJD92_RS04255	733765..734271	+	507	WP_000228937.1	G/U mismatch-specific DNA glycosylase	-
GJD92_RS04260	734349..736190	-	1842	WP_000437371.1	RNA polymerase sigma factor RpoD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (13-112)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13932.92 Da
• Isoelectric Point: 8.5163

>T143273 WP_154139486.1 NZ_CP046000:731739-732116 [Escherichia coli]
MKTLPVLPGQSASSRPSPVEIWQTLLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPGAKQ

Nucleotide

• Length: 378 bp

>T143273 NZ_CP060987:185984-186091 [Escherichia coli]
ATGACGTTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin

Protein

• Length: 118 a.a.
• Molecular weight: 13130.84 Da
• Isoelectric Point: 6.3160

>AT143273 WP_174213414.1 NZ_CP046000:731296-731649 [Escherichia coli]
VSDTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADANHLDQAFPLLMKQLELMLTSSELNPR
HQHTVTLYARGLTCEADTLGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 354 bp

>AT143273 NZ_CP060987:c185936-185870 [Escherichia coli]
GGTCTAGAGTCAAGATTAGCCCCCGTGGTGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTTCT