Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) ataRT/DUF1778(antitoxin)
Location 325207..326007 Replicon chromosome
Accession NZ_CP046000
Organism Escherichia coli strain 1916D18

Toxin (Protein)


Gene name ataT Uniprot ID F4NNI0
Locus tag GJD92_RS02220 Protein ID WP_000342449.1
Coordinates 325480..326007 (+) Length 176 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID F4NNI1
Locus tag GJD92_RS02215 Protein ID WP_001277108.1
Coordinates 325207..325473 (+) Length 89 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GJD92_RS02195 320865..321533 + 669 WP_000617723.1 cell division ATP-binding protein FtsE -
GJD92_RS02200 321526..322584 + 1059 WP_001042003.1 permease-like cell division protein FtsX -
GJD92_RS02205 322829..323683 + 855 WP_000130221.1 RNA polymerase sigma factor RpoH -
GJD92_RS02210 323954..325057 + 1104 WP_001350438.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
GJD92_RS02215 325207..325473 + 267 WP_001277108.1 DUF1778 domain-containing protein Antitoxin
GJD92_RS02220 325480..326007 + 528 WP_000342449.1 GNAT family N-acetyltransferase Toxin
GJD92_RS02225 326004..326387 - 384 WP_000778781.1 aspartate 1-decarboxylase autocleavage activator PanM -
GJD92_RS02230 326811..327920 + 1110 WP_000827696.1 high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK -
GJD92_RS02235 327968..328894 + 927 WP_000003004.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
GJD92_RS02240 328891..330168 + 1278 WP_000803799.1 branched chain amino acid ABC transporter permease LivM -
GJD92_RS02245 330165..330932 + 768 WP_000082101.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(88-150)

Antitoxin

(8-86)


Sequences


Toxin        


Download         Length: 176 a.a.        Molecular weight: 19691.70 Da        Isoelectric Point: 7.7457

>T143271 WP_000342449.1 NZ_CP046000:325480-326007 [Escherichia coli]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLCGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDRSLQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNKKAHTFYQSLGFIPLVGENENAL
FFPTKSIELLFTQSD

Download         Length: 528 bp

>T143271 NZ_CP060983:5142-5393 [Escherichia coli]
ATGAGAAAAAATAAAGGTGATGTGACTTATTTTCTTGAAAAAGAAGGTGACAATTACAGGCTTACAAAAAGGATAAAAGC
CAGAACAAATGTTAAAATCGGGAACAAAACTACGAAAATTACATTGTATGATGCCGTACTGAATGAGAATGAATTACAAC
ATATTGATTTTACGTGTGCCGGATTAAGGGAAGACGATGAGACGCCAGTTAAGAATTTAATTAAGGAGTTTATGTTAAAT
GAAACCCGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9910.31 Da        Isoelectric Point: 10.0863

>AT143271 WP_001277108.1 NZ_CP046000:325207-325473 [Escherichia coli]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRVILNEESWTRVMDALSNPPSPGEKLKR
AAKRLQGM

Download         Length: 267 bp

>AT143271 NZ_CP060983:5380-5607 [Escherichia coli]
ATGAAACCCGATAATACACCTGAAAATGTAAAAAAATTACGTTTAAAAGCAGGATTAACACAGAAAGAATGCAGTCATAT
TTATGGTGTTGGGCTAAGAACCTGGCAGAAAAAAGAGGAGGTTAATACTCAAAACAGTCAAAGTCTGTCACTGGTTGAGT
TTGAGTTTCTTTTGTTACTTGCAGGAGAACATCCTGAATACGTGCTCTGCAAAAGGGAGTCAAAATAA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB A0A829CLZ4


Antitoxin

Source ID Structure
PDB 6GTS
PDB 6AJN
PDB 6GTQ
PDB 6GTO
PDB 6GTR
PDB 6AJM
AlphaFold DB A0A829CN24

References