Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 68786..69429 Replicon plasmid p1916D18-2
Accession NZ_CP045999
Organism Escherichia coli strain 1916D18

Toxin (Protein)


Gene name vagD Uniprot ID -
Locus tag GJD92_RS00645 Protein ID WP_063073410.1
Coordinates 68786..69202 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID A0A3Q0N5I0
Locus tag GJD92_RS00650 Protein ID WP_063073411.1
Coordinates 69199..69429 (-) Length 77 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GJD92_RS00615 64038..64691 + 654 Protein_53 IS21-like element IS21 family transposase -
GJD92_RS00620 64691..65475 + 785 Protein_54 ATP-binding protein -
GJD92_RS00625 65534..65629 - 96 Protein_55 VapC toxin family PIN domain ribonuclease -
GJD92_RS00630 65626..65856 - 231 WP_001261276.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
GJD92_RS00635 66143..67165 - 1023 WP_063073408.1 hypothetical protein -
GJD92_RS00640 67150..68712 - 1563 WP_063073409.1 AAA family ATPase -
GJD92_RS00645 68786..69202 - 417 WP_063073410.1 type II toxin-antitoxin system VapC family toxin Toxin
GJD92_RS00650 69199..69429 - 231 WP_063073411.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
GJD92_RS23875 69386..69565 + 180 Protein_61 hypothetical protein -
GJD92_RS00655 69709..70413 - 705 WP_154139461.1 IS6 family transposase -
GJD92_RS00660 70479..70910 + 432 WP_001707302.1 hypothetical protein -
GJD92_RS00665 70907..71698 + 792 WP_000016494.1 site-specific integrase -
GJD92_RS00670 71850..72125 - 276 WP_050858693.1 hypothetical protein -
GJD92_RS00675 72119..72763 - 645 WP_000633913.1 AAA family ATPase -
GJD92_RS00680 72992..73963 + 972 WP_001103690.1 hypothetical protein -
GJD92_RS00685 73968..74360 + 393 WP_063073471.1 plasmid partitioning/stability family protein -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid tet(A) - 1..78498 78498
- inside IScluster/Tn - - 61705..70413 8708


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(5-129)

Antitoxin

(7-51)


Sequences


Toxin        


Download         Length: 139 a.a.        Molecular weight: 15167.53 Da        Isoelectric Point: 7.8882

>T143269 WP_063073410.1 NZ_CP045999:c69202-68786 [Escherichia coli]
VNKTYMLDTNICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCTRLDAILPWDRA
AVDATTEIKVALRLAGTPIGPNDTAIAGHSIAAGAVLVTNNTREFERVPGLVLEDWIR

Download         Length: 417 bp

>T143269 NZ_CP060981:61462-61614 [Escherichia coli]
ATGCCACAGCGAACGTTTTTAATGATGTTAATCGTCATCTGTGTGACGATTCTGTGTTTTGTCTGGATGGTGAGGGATTC
GCTTTGCGGACTCCGGCTCCAGCAGGGAAACACAGTGCTTGTGGCAACGTTAGCCTACGAAGTTAAACGTTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8688.84 Da        Isoelectric Point: 4.6310

>AT143269 WP_063073411.1 NZ_CP045999:c69429-69199 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAKLEKADPDFMAEREDVVTDEGRVNL

Download         Length: 231 bp

>AT143269 NZ_CP060981:c61412-61350 [Escherichia coli]
TCGAAGCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure


Antitoxin

Source ID Structure
AlphaFold DB A0A3Q0N5I0

References