Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 41232..41757 Replicon plasmid p1916D18-2
Accession NZ_CP045999
Organism Escherichia coli strain 1916D18

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag GJD92_RS00525 Protein ID WP_001159871.1
Coordinates 41452..41757 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag GJD92_RS00520 Protein ID WP_000813634.1
Coordinates 41232..41450 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GJD92_RS00510 38644..39827 + 1184 Protein_32 IS3 family transposase -
GJD92_RS00515 40126..40416 + 291 WP_000990619.1 hypothetical protein -
GJD92_RS00520 41232..41450 + 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
GJD92_RS00525 41452..41757 + 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
GJD92_RS00530 41758..42564 + 807 WP_020219156.1 site-specific integrase -
GJD92_RS00535 42639..43852 + 1214 WP_162829202.1 IS3 family transposase -
GJD92_RS00540 44096..44788 - 693 WP_020219155.1 AAA family ATPase -
GJD92_RS00545 44788..45288 - 501 Protein_39 IS21-like element IS100 family transposase -
GJD92_RS00550 45341..45911 + 571 Protein_40 IS3 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid tet(A) - 1..78498 78498
- inside IScluster/Tn tet(A) - 12400..53688 41288


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T143268 WP_001159871.1 NZ_CP045999:41452-41757 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T143268 NZ_CP060981:2255-2533 [Escherichia coli]
ATGGAGATATTCTGGACCATGCTGGCCAGCCAGGACAGGAAGCGCATTCGTAAGTACATTGCGGAGCAGAACCTGATGGC
CGCCATTGAACTGGACGAACGGATTGGTTATTCAGCCAGCAGCCTGGCCGGGCAGCCGTATAAAGGCCGCAACGGTCGTG
TGGAAGGTACCCGGGAACTGGTTATTCACCCGCATTTTGTTCTGGTTTATGAGGTCGACAGCCAGTGGGGAAAAGTGTAT
ATCCTGCGCGTGTTGCATACTGCACAGAAGTGGCCATAG

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT143268 WP_000813634.1 NZ_CP045999:41232-41450 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT143268 NZ_CP060981:1986-2255 [Escherichia coli]
ATGGCACAGGTTAATATGAGTTTAAGAATCGACGCTGAACTGAAGGATGCTTTTATGGCCGCTGCAAAAAGCATGGACCG
TAATGGCTCTCAGTTAATTCGGGATTTTATGCGCCAGACCGTTGAACGGCAGCATAATACCTGGTTCCGTGACCAGGTTG
AGGCAGGACGTCAGCAACTCGAGCGCGGCGATGTGCTTCCCCATGACATGGTCGAATCTTCCGCTGCTGCATGGCGGGAT
GAAATGAGCAGGAAGGTTGCCGGTAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References