Detailed information of TA system TA143265

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/upstrm_HI1419-dnstrm_HI1420
• Location: 2656938..2657579
• Replicon: chromosome
• Accession: NZ_CP045995
• Organism: Burkholderia sp. MS389

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: GJG85_RS28275
• Protein ID: WP_204384121.1
• Coordinates: 2657241..2657579 (-)
• Length: 113 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A228HNU1
• Locus tag: GJG85_RS28270
• Protein ID: WP_050017389.1
• Coordinates: 2656938..2657237 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GJG85_RS28245	2652170..2653441	+	1272	WP_050017393.1	Rieske 2Fe-2S domain-containing protein	-
GJG85_RS28250	2653456..2653932	+	477	WP_006479528.1	aromatic-ring-hydroxylating dioxygenase subunit beta	-
GJG85_RS28255	2653950..2654276	+	327	WP_124553930.1	non-heme iron oxygenase ferredoxin subunit	-
GJG85_RS28260	2654266..2655486	+	1221	WP_204384120.1	FAD-dependent oxidoreductase	-
GJG85_RS28265	2655598..2656884	+	1287	WP_069618588.1	DUF445 domain-containing protein	-
GJG85_RS28270	2656938..2657237	-	300	WP_050017389.1	putative addiction module antidote protein	Antitoxin
GJG85_RS28275	2657241..2657579	-	339	WP_204384121.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GJG85_RS28280	2657963..2659603	+	1641	WP_089489109.1	acetolactate synthase large subunit	-
GJG85_RS28285	2659632..2661065	+	1434	WP_204384123.1	aldehyde dehydrogenase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (16-107)

Antitoxin

• Uncharacterized protein HI1420 (6-93)

Sequences

Toxin

Protein

• Length: 113 a.a.
• Molecular weight: 12662.56 Da
• Isoelectric Point: 9.4963

>T143265 WP_204384121.1 NZ_CP045995:c2657579-2657241 [Burkholderia sp. MS389]
MVDILQSVPYSAPTYSIRTTDVFDVWFAGLPDRLARRRIQARIDRLSMGNPGDWKSAGSPVVEMRIDHGPGYRIYYARRG
TIWVILLCGGDKSTQRADIRAAHAMLAHLDME

Nucleotide

• Length: 339 bp

>T143265 NZ_CP060979:c4198253-4198080 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 10593.97 Da
• Isoelectric Point: 6.0669

>AT143265 WP_050017389.1 NZ_CP045995:c2657237-2656938 [Burkholderia sp. MS389]
MDKINTRPWDSAEHLKTEDDMAEYFDACLQEGGDDPAFIAHALGVIARARGMSQVARDAGLSREGLYKALSHDGNPSFGT
ILKVIKALGLQLHGAKAAA

Nucleotide

• Length: 300 bp

>AT143265 NZ_CP060979:c4198577-4198281 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGATT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Structures

Antitoxin

• AlphaFold DB: A0A228HNU1