Detailed information of TA system TA143263

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 1852834..1853353
• Replicon: chromosome
• Accession: NZ_CP045995
• Organism: Burkholderia sp. MS389

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A8E2SU86
• Locus tag: GJG85_RS24630
• Protein ID: WP_050018268.1
• Coordinates: 1852834..1853115 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A108LKY6
• Locus tag: GJG85_RS24635
• Protein ID: WP_048023838.1
• Coordinates: 1853105..1853353 (-)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GJG85_RS24605	1847923..1848474	+	552	WP_124631379.1	YbhB/YbcL family Raf kinase inhibitor-like protein	-
GJG85_RS24610	1848598..1849296	-	699	WP_050018272.1	MgtC/SapB family protein	-
GJG85_RS24615	1849410..1850519	+	1110	WP_176016298.1	AGE family epimerase/isomerase	-
GJG85_RS24620	1850492..1852111	-	1620	WP_050018270.1	Tar ligand binding domain-containing protein	-
GJG85_RS24625	1852362..1852805	+	444	WP_050018269.1	PaaI family thioesterase	-
GJG85_RS24630	1852834..1853115	-	282	WP_050018268.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GJG85_RS24635	1853105..1853353	-	249	WP_048023838.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
GJG85_RS24640	1853482..1854534	-	1053	WP_050013339.1	CoA transferase	-
GJG85_RS24645	1854695..1855459	-	765	WP_050013340.1	helix-turn-helix domain-containing protein	-
GJG85_RS24650	1855594..1856376	+	783	WP_050013341.1	crotonase/enoyl-CoA hydratase family protein	-
GJG85_RS24655	1856457..1857677	+	1221	WP_050013342.1	CoA transferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (7-84)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10908.82 Da
• Isoelectric Point: 10.6768

>T143263 WP_050018268.1 NZ_CP045995:c1853115-1852834 [Burkholderia sp. MS389]
MTFELAFLEPALKEWKKLDRTVRDQFKARLAERLEHPRIPSAKLHGHPDRDKIKLRGVGYRLVYEVRDAEVIVLVVAVGR
RERDAVYLAAMKR

Nucleotide

• Length: 282 bp

>T143263 NZ_CP060979:3654893-3655171 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATATTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 8963.31 Da
• Isoelectric Point: 5.0636

>AT143263 WP_048023838.1 NZ_CP045995:c1853353-1853105 [Burkholderia sp. MS389]
MPHTILASVTASVSELKRNPMGTVAAGEGFPVAILNHNEPAFYCIPAKTWEAMVERLEDIEDNALADSRAKEKVVKVKLN
DL

Nucleotide

• Length: 249 bp

>AT143263 NZ_CP060979:3654630-3654890 [Escherichia coli]
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCAGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCATTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Structures

Antitoxin

• AlphaFold DB: A0A108LKY6