Detailed information of TA system TA143262

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 1642151..1642716
• Replicon: chromosome
• Accession: NZ_CP045995
• Organism: Burkholderia sp. MS389

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A8E2MXQ6
• Locus tag: GJG85_RS23690
• Protein ID: WP_050017312.1
• Coordinates: 1642345..1642716 (+)
• Length: 124 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: B1FGI9
• Locus tag: GJG85_RS23685
• Protein ID: WP_006752328.1
• Coordinates: 1642151..1642348 (+)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GJG85_RS23660	1637444..1637899	-	456	WP_050017306.1	RbsD/FucU family protein	-
GJG85_RS23665	1637979..1638371	-	393	WP_089489800.1	nuclear transport factor 2 family protein	-
GJG85_RS23670	1638597..1640150	-	1554	WP_050017308.1	galactarate dehydratase	-
GJG85_RS23675	1640380..1641294	+	915	WP_089489801.1	5-dehydro-4-deoxyglucarate dehydratase	-
GJG85_RS23680	1641411..1641965	-	555	WP_050017310.1	hypothetical protein	-
GJG85_RS23685	1642151..1642348	+	198	WP_006752328.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
GJG85_RS23690	1642345..1642716	+	372	WP_050017312.1	PIN domain-containing protein	Toxin
GJG85_RS23695	1642844..1644421	-	1578	WP_089489802.1	aldehyde dehydrogenase (NADP(+))	-
GJG85_RS23700	1644668..1646053	+	1386	WP_050017314.1	MFS transporter	-
GJG85_RS23705	1646120..1646845	-	726	WP_050017315.1	site-2 protease family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-112)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-46)

Sequences

Toxin

Protein

• Length: 124 a.a.
• Molecular weight: 13593.73 Da
• Isoelectric Point: 6.3291

>T143262 WP_050017312.1 NZ_CP045995:1642345-1642716 [Burkholderia sp. MS389]
MILVDTSVWIDHLRAGDATLTALLEAERVLIHPFIVGELALRSMRNRETVLDALRDLPAATVATDDEVQRMIDAVPLHGL
GIGYVDAHLLASARLTSDSKLWTRDRRLLAAAERLQLVARPAH

Nucleotide

• Length: 372 bp

>T143262 NZ_CP060979:c3648831-3648433 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCCTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 7054.10 Da
• Isoelectric Point: 10.6826

>AT143262 WP_006752328.1 NZ_CP045995:1642151-1642348 [Burkholderia sp. MS389]
MRTTLSLDDALLAKAQQLTGVTEKSALVREALRALIARESARRLARLGGTEPDLESVPRRPSEPA

Nucleotide

• Length: 198 bp

>AT143262 NZ_CP060979:c3649127-3648834 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Antitoxin

• AlphaFold DB: A0A2S9KMB8