Detailed information of TA system TA143258

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-StbC
• Location: 590300..590939
• Replicon: chromosome
• Accession: NZ_CP045995
• Organism: Burkholderia sp. MS389

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: GJG85_RS19130
• Protein ID: WP_050014113.1
• Coordinates: 590300..590710 (-)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A8E2N1X6
• Locus tag: GJG85_RS19135
• Protein ID: WP_050014174.1
• Coordinates: 590703..590939 (-)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GJG85_RS19115	587085..588464	+	1380	WP_050014112.1	MFS transporter	-
GJG85_RS19120	588971..589234	-	264	WP_006483034.1	translation initiation factor IF-1	-
GJG85_RS19125	589699..589902	-	204	WP_006483056.1	cold-shock protein	-
GJG85_RS19130	590300..590710	-	411	WP_050014113.1	type II toxin-antitoxin system VapC family toxin	Toxin
GJG85_RS19135	590703..590939	-	237	WP_050014174.1	DNA-binding protein	Antitoxin
GJG85_RS19140	591449..592636	+	1188	Protein_516	acyltransferase	-
GJG85_RS19145	592818..594206	+	1389	WP_050014114.1	DHA2 family efflux MFS transporter permease subunit	-
GJG85_RS19150	594216..594596	-	381	WP_089489398.1	DUF4377 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-128)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 15242.50 Da
• Isoelectric Point: 6.8819

>T143258 WP_050014113.1 NZ_CP045995:c590710-590300 [Burkholderia sp. MS389]
MIDTNVISEIRKGRRTNRGVRAFFRQAEAEASPLYLSVVTVAELRRGVDLIRHRGDHPQASALEAWMAMIVSGYAPNILP
VDIEISQMWGHLRVPDPTHELDKLIAATALINDLTVVTRNVDDFARTGVRLLNPFD

Nucleotide

• Length: 411 bp

>T143258 NZ_CP060979:c2419334-2419158 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATAGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8655.70 Da
• Isoelectric Point: 4.7672

>AT143258 WP_050014174.1 NZ_CP045995:c590939-590703 [Burkholderia sp. MS389]
MANLLVRNVDDTIVQSLREQAAANGRSAEAEHRAILAEALGRPKRRTFAQVLMSMPEVGEDADFQRVQDSGEVTRVFD

Nucleotide

• Length: 237 bp

>AT143258 NZ_CP060979:c2419112-2418696 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA