Detailed information of TA system TA143256

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): spoIISABC/SpoIISA-SpoIISB
• Location: 1482609..1483526
• Replicon: chromosome
• Accession: NZ_CP045993
• Organism: Bacillus velezensis strain LABIM22

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: I2HQ15
• Locus tag: GJS30_RS07670
• Protein ID: WP_007407256.1
• Coordinates: 1482780..1483526 (-)
• Length: 249 a.a.

Antitoxin (Protein)

• Gene name: spoIISB
• Uniprot ID: I2HQ14
• Locus tag: GJS30_RS07665
• Protein ID: WP_003154807.1
• Coordinates: 1482609..1482779 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GJS30_RS07630	1477821..1479410	+	1590	WP_021493824.1	hypothetical protein	-
GJS30_RS07635	1479423..1479848	+	426	WP_021493823.1	hypothetical protein	-
GJS30_RS07640	1479853..1480050	+	198	WP_007610833.1	XkdX family protein	-
GJS30_RS07645	1480107..1480868	+	762	WP_179156004.1	phage portal protein	-
GJS30_RS07650	1480920..1481183	+	264	WP_021493821.1	hemolysin XhlA family protein	-
GJS30_RS07655	1481197..1481460	+	264	WP_021493820.1	phage holin	-
GJS30_RS07660	1481474..1482352	+	879	WP_007407257.1	N-acetylmuramoyl-L-alanine amidase	-
GJS30_RS07665	1482609..1482779	-	171	WP_003154807.1	type II toxin-antitoxin system SpoIISB family antitoxin	Antitoxin
GJS30_RS07670	1482780..1483526	-	747	WP_007407256.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
GJS30_RS07675	1483630..1484628	-	999	WP_003154805.1	inorganic phosphate transporter	-
GJS30_RS07680	1484641..1485258	-	618	WP_003154804.1	DUF47 domain-containing protein	-
GJS30_RS07685	1485544..1486860	-	1317	WP_007610842.1	amino acid permease	-
GJS30_RS07690	1487182..1488132	+	951	WP_007610844.1	ring-cleaving dioxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1436837..1541576	104739

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (3-239)

Antitoxin

• Antitoxin SpoIISB (1-56)

Sequences

Toxin

Protein

• Length: 249 a.a.
• Molecular weight: 29077.57 Da
• Isoelectric Point: 4.7755

>T143256 WP_007407256.1 NZ_CP045993:c1483526-1482780 [Bacillus velezensis]
MLLFFQIMVWTMAAALILYVYASWRYEAKVKEKMFAIRKTWYLLFVLGSMVYWTYDPESLFAAWRQYLIVAVCFALIDAF
IFLSAYIKKLAGNELETDTREILEENNEMLHSYLEKLKTYQYLLKNEPIHVYYGSTEAYAEGISRLLAAYAEKMNVTASL
CDYSAQSDKDRLTEHMPDAADVQSRLNRKDVYYDQKGRLVLIPFTVQNRHYVIKLTSENLLTEFDYLLFTSLTSIYDLML
PIEEEGDG

Nucleotide

• Length: 747 bp

>T143256 NZ_CP060979:2281351-2281638 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6676.74 Da
• Isoelectric Point: 10.9273

>AT143256 WP_003154807.1 NZ_CP045993:c1482779-1482609 [Bacillus velezensis]
MEQAFQNGQPEQRAKPFKMFKKRSMTSIASYQVSPHTARIFKENERLIDKYKQKKA

Nucleotide

• Length: 171 bp

>AT143256 NZ_CP060979:2281112-2281351 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Structures

Toxin

• AlphaFold DB: I2HQ15

Antitoxin

• AlphaFold DB: I2HQ14