Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 721935..722572 Replicon chromosome
Accession NZ_CP045993
Organism Bacillus velezensis strain LABIM22

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag GJS30_RS03615 Protein ID WP_003156187.1
Coordinates 722222..722572 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID I2HMS5
Locus tag GJS30_RS03610 Protein ID WP_003156188.1
Coordinates 721935..722216 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GJS30_RS03590 718300..718899 - 600 WP_053573675.1 rhomboid family intramembrane serine protease -
GJS30_RS03595 718992..719357 + 366 WP_014304402.1 holo-ACP synthase -
GJS30_RS03600 719522..720529 + 1008 WP_021495080.1 outer membrane lipoprotein carrier protein LolA -
GJS30_RS03605 720646..721815 + 1170 WP_053285486.1 alanine racemase -
GJS30_RS03610 721935..722216 + 282 WP_003156188.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
GJS30_RS03615 722222..722572 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
GJS30_RS03620 722690..723511 + 822 WP_003156182.1 STAS domain-containing protein -
GJS30_RS03625 723516..723881 + 366 WP_003156180.1 RsbT antagonist protein RsbS -
GJS30_RS03630 723884..724285 + 402 WP_003156178.1 anti-sigma regulatory factor -
GJS30_RS03635 724297..725304 + 1008 WP_007609589.1 PP2C family protein-serine/threonine phosphatase -
GJS30_RS03640 725368..725697 + 330 WP_003156176.1 anti-sigma factor antagonist RsbV -
GJS30_RS03645 725694..726176 + 483 WP_007609591.1 anti-sigma B factor RsbW -
GJS30_RS03650 726142..726930 + 789 WP_003156171.1 RNA polymerase sigma factor SigB -
GJS30_RS03655 726930..727532 + 603 WP_007410234.1 SpoIIE family protein phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T143255 WP_003156187.1 NZ_CP045993:722222-722572 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

>T143255 NZ_CP060979:1999758-1999861 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10562.92 Da        Isoelectric Point: 6.4706

>AT143255 WP_003156188.1 NZ_CP045993:721935-722216 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Download         Length: 282 bp

>AT143255 NZ_CP060979:c1999899-1999628 [Escherichia coli]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTGAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
AlphaFold DB I2HMS5

References