Detailed information of TA system TA143253

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 5791..6316
• Replicon: plasmid pCTXM15_020035
• Accession: NZ_CP045989
• Organism: Klebsiella pneumoniae strain WCHKP35

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: D4HQE7
• Locus tag: CEA74_RS25635
• Protein ID: WP_013023785.1
• Coordinates: 5791..6096 (-)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: W8V2V6
• Locus tag: CEA74_RS25640
• Protein ID: WP_001568025.1
• Coordinates: 6098..6316 (-)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CEA74_RS25605 (1518)	1518..2144	+	627	WP_004197649.1	ParA family plasmid-partitioning AAA ATPase	-
CEA74_RS25610 (2141)	2141..2443	+	303	WP_004197636.1	hypothetical protein	-
CEA74_RS25615 (2867)	2867..3661	-	795	WP_004197635.1	site-specific integrase	-
CEA74_RS25620 (3859)	3859..4875	-	1017	WP_017899884.1	hypothetical protein	-
CEA74_RS25625 (4886)	4886..5200	-	315	WP_053389906.1	hypothetical protein	-
CEA74_RS25630 (5227)	5227..5622	-	396	WP_017899885.1	hypothetical protein	-
CEA74_RS25635 (5791)	5791..6096	-	306	WP_013023785.1	type II toxin-antitoxin system toxin CcdB	Toxin
CEA74_RS25640 (6098)	6098..6316	-	219	WP_001568025.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
CEA74_RS25645 (6871)	6871..7560	+	690	WP_004193995.1	hypothetical protein	-
CEA74_RS25650 (7592)	7592..8281	-	690	WP_020315560.1	RES family NAD+ phosphorylase	-
CEA74_RS25655 (8718)	8718..8966	+	249	WP_000272716.1	hypothetical protein	-
CEA74_RS25660 (8963)	8963..9535	+	573	WP_020805590.1	hypothetical protein	-
CEA74_RS25665 (9566)	9566..10060	+	495	WP_020805591.1	hypothetical protein	-
CEA74_RS25670 (10104)	10104..10472	+	369	WP_004197807.1	hypothetical protein	-
CEA74_RS25675 (10506)	10506..10709	+	204	WP_004197808.1	HHA domain-containing protein	-
CEA74_RS25680 (10723)	10723..10926	+	204	WP_004197809.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	qnrS1 / blaCTX-M-15 / aac(6')-Ib / ant(3'')-Ia / blaOXA-9 / aac(3)-IId	-	1..106446	106446

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11615.30 Da
• Isoelectric Point: 6.4672

>T143253 WP_013023785.1 NZ_CP045989:c6096-5791 [Klebsiella pneumoniae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESYRLMTTDMASVTSSVTGEEV
ADLSHRENDIKNAINLMFWGI

Nucleotide

• Length: 306 bp

>T143253 NZ_CP060979:1794295-1794549 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCTCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCTACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8392.34 Da
• Isoelectric Point: 4.7405

>AT143253 WP_001568025.1 NZ_CP045989:c6316-6098 [Klebsiella pneumoniae]
MKQRITVTIDSDSYQLLKSADVNISALVNRAMQNEARRLRSERWQAENQEGMAEVARFIEMNGSFADENRDW

Nucleotide

• Length: 219 bp

>AT143253 NZ_CP060979:1794047-1794298 [Escherichia coli]
ATGCGTACAATTAGTTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Structures

Toxin

• AlphaFold DB: A0A2J4ZZW6

Antitoxin

• AlphaFold DB: A0A2J4ZZP8