Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5205944..5206569 Replicon chromosome
Accession NZ_CP045988
Organism Klebsiella pneumoniae strain WCHKP35

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag CEA74_RS25235 Protein ID WP_002882817.1
Coordinates 5205944..5206327 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag CEA74_RS25240 Protein ID WP_004150355.1
Coordinates 5206327..5206569 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CEA74_RS25220 (5203310) 5203310..5204212 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
CEA74_RS25225 (5204209) 5204209..5204844 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
CEA74_RS25230 (5204841) 5204841..5205770 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
CEA74_RS25235 (5205944) 5205944..5206327 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
CEA74_RS25240 (5206327) 5206327..5206569 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
CEA74_RS25245 (5206774) 5206774..5207691 + 918 WP_009484979.1 alpha/beta hydrolase -
CEA74_RS25250 (5207705) 5207705..5208646 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
CEA74_RS25255 (5208691) 5208691..5209128 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
CEA74_RS25260 (5209125) 5209125..5209985 - 861 WP_004146232.1 virulence factor BrkB family protein -
CEA74_RS25265 (5209979) 5209979..5210578 - 600 WP_087497883.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T143252 WP_002882817.1 NZ_CP045988:c5206327-5205944 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T143252 NZ_CP060979:1794047-1794298 [Escherichia coli]
ATGCGTACAATTAGTTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT143252 WP_004150355.1 NZ_CP045988:c5206569-5206327 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT143252 NZ_CP060979:1794295-1794549 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCTCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCTACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References