Detailed information of TA system TA143247

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 3985138..3985757
• Replicon: chromosome
• Accession: NZ_CP045988
• Organism: Klebsiella pneumoniae strain WCHKP35

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: R8WYV2
• Locus tag: CEA74_RS19365
• Protein ID: WP_002892050.1
• Coordinates: 3985539..3985757 (+)
• Length: 73 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: -
• Locus tag: CEA74_RS19360
• Protein ID: WP_080789174.1
• Coordinates: 3985138..3985512 (+)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CEA74_RS19350 (3980290)	3980290..3981483	+	1194	WP_004177236.1	multidrug efflux RND transporter periplasmic adaptor subunit AcrA	-
CEA74_RS19355 (3981506)	3981506..3984652	+	3147	WP_002892069.1	multidrug efflux RND transporter permease subunit AcrB	-
CEA74_RS19360 (3985138)	3985138..3985512	+	375	WP_080789174.1	Hha toxicity modulator TomB	Antitoxin
CEA74_RS19365 (3985539)	3985539..3985757	+	219	WP_002892050.1	HHA domain-containing protein	Toxin
CEA74_RS19370 (3985916)	3985916..3986482	+	567	WP_002892030.1	maltose O-acetyltransferase	-
CEA74_RS19375 (3986454)	3986454..3986594	-	141	WP_004147370.1	hypothetical protein	-
CEA74_RS19380 (3986615)	3986615..3987085	+	471	WP_002892026.1	YlaC family protein	-
CEA74_RS19385 (3987060)	3987060..3988510	-	1451	Protein_3808	PLP-dependent aminotransferase family protein	-
CEA74_RS19390 (3988611)	3988611..3989309	+	699	WP_002892021.1	GNAT family protein	-
CEA74_RS19395 (3989306)	3989306..3989446	-	141	WP_002892018.1	type B 50S ribosomal protein L36	-
CEA74_RS19400 (3989446)	3989446..3989709	-	264	WP_002892011.1	type B 50S ribosomal protein L31	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (13-69)

Antitoxin

• Biofilm formation regulator YbaJ (1-116)

Sequences

Toxin

Protein

• Length: 73 a.a.
• Molecular weight: 8628.00 Da
• Isoelectric Point: 8.9008

>T143247 WP_002892050.1 NZ_CP045988:3985539-3985757 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Nucleotide

• Length: 219 bp

>T143247 NZ_CP060979:c617416-616952 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAGACAGAAGAAACCCATTGA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 14295.99 Da
• Isoelectric Point: 5.1444

>AT143247 WP_080789174.1 NZ_CP045988:3985138-3985512 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAGDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Nucleotide

• Length: 375 bp

>AT143247 NZ_CP060979:c617751-617416 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Structures

Toxin

• AlphaFold DB: A0A2P8K6F2